In Vivo Study of Monoamine Oxidases Using Multisite Intracerebral Microdialysis
Methods in Molecular Biology. 2022-09-29; : 183-195
DOI: 10.1007/978-1-0716-2643-6_14
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1. Methods Mol Biol. 2023;2558:183-195. doi: 10.1007/978-1-0716-2643-6_14.
In Vivo Study of Monoamine Oxidases Using Multisite Intracerebral Microdialysis.
De Deurwaerdère P(1), Samb N(2), El Boukhari H(2), Corne R(2), Chagraoui
A(3)(4), Di Giovanni G(5)(6).
Author information:
(1)Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5287),
Bordeaux Cedex, France. .
(2)Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5287),
Bordeaux Cedex, France.
(3)Normandie University, UNIROUEN, INSERM, U1239, CHU Rouen, Neuronal and
Neuroendocrine Differentiation and Communication Laboratory, Institute for
Research and Innovation in Biomedicine of Normandy (IRIB), Rouen, France.
(4)Department of Medical Biochemistry, Rouen University Hospital, Rouen, France.
(5)Department of Physiology and Biochemistry, Faculty of Medicine and Surgery,
University of Malta, Msida, Malta.
(6)School of Biosciences, Cardiff University, Cardiff, UK.
The activity of monoamine oxidases (MAOs) in the brain is often associated with
neurodegenerative diseases. The study of MAOs in vivo or ex vivo is generally
performed using MAO inhibitors and rarely using substrates. We present a
pharmacological approach using intracerebral microdialysis to study the activity
of MAO in the striatum and the prefrontal cortex of rats. It consists of
applying ascending concentrations of 3-methoxytyramine (3-MT) as a substrate via
the probes and measuring the indirect product homovanillic acid generated by MAO
activity. We present herein the methodologies comprising our in-house
stereotaxic procedures in rats, the microdialysis perfusion system and the
substrate application, and the neurochemical analysis of the samples.
© 2023. The Author(s), under exclusive license to Springer Science+Business
Media, LLC, part of Springer Nature.
DOI: 10.1007/978-1-0716-2643-6_14
PMID: 36169864 [Indexed for MEDLINE]