Calcium Signaling in Isolated Skeletal Muscle Fibers Investigated Under « Silicone Voltage-Clamp » Conditions
CBB. 2004-01-01; 40(2): 225-236
DOI: 10.1385/CBB:40:2:225
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1. Cell Biochem Biophys. 2004;40(2):225-36. doi: 10.1385/CBB:40:2:225.
Calcium signaling in isolated skeletal muscle fibers investigated under
« Silicone Voltage-Clamp » conditions.
Collet C(1), Pouvreau S, Csernoch L, Allard B, Jacquemond V.
Author information:
(1)Laboratoire de Physiologie des Eléments Excitables, Université Claude Bernard
Lyon 1, UMR CNRS 5123, F69622 Villeurbanne, France.
In skeletal muscle, release of calcium from the sarcoplasmic reticulum (SR)
represents the major source of cytoplasmic Ca2+ elevation. SR calcium release is
under the strict command of the membrane potential, which drives the interaction
between the voltage sensors in the t-tubule membrane and the calcium-release
channels. Either detection or control of the membrane voltage is thus essential
when studying intracellular calcium signaling in an intact muscle fiber
preparation. The silicone-clamp technique used in combination with intracellular
calcium measurements represents an efficient tool for such studies. This article
reviews some properties of the plasma membrane and intracellular signals
measured with this methodology in mouse skeletal muscle fibers. Focus is given
to the potency of this approach to investigate both fundamental aspects of
excitation-contraction coupling and potential alterations of intracellular
calcium handling in some muscle diseases.
DOI: 10.1385/CBB:40:2:225
PMID: 15054224 [Indexed for MEDLINE]