Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells
Biomaterials Advances. 2024-05-01; 159: 213819
DOI: 10.1016/j.bioadv.2024.213819
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Klimovič Š(1), Beckerová D(2), Věžník J(3), Kabanov D(1), Lacina K(4), Jelinkova S(5), Gumulec J(6), Rotrekl V(2), Přibyl J(7).
Author information:
(1)CEITEC, Masaryk University, Brno, Czech Republic; Department of Biochemistry,
Faculty of Science, Masaryk University, Brno, Czech Republic.
(2)Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech
Republic; ICRC, St. Anne’s University Hospital, Brno, Czech Republic.
(3)CEITEC, Masaryk University, Brno, Czech Republic; Department of Chemistry,
Faculty of Science, Masaryk University, Brno, Czech Republic.
(4)CEITEC, Masaryk University, Brno, Czech Republic.
(5)Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech
Republic.
(6)Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno,
Czech Republic.
(7)CEITEC, Masaryk University, Brno, Czech Republic. Electronic address:
.
Extracellular matrix (ECM) regulates cellular responses through
mechanotransduction. The standard approach of in vitro culturing on plastic
surfaces overlooks this phenomenon, so there is a need for biocompatible
materials that exhibit adjustable mechanical and structural properties, promote
cell adhesion and proliferation at low cost and for use in 2D or 3D cell
cultures. This study presents a new tunable hydrogel system prepared from
high-molecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin
cross-linked using EDC/NHS. Hydrogels with Young’s moduli (E) ranging from
subunit to units of kilopascals were prepared by gradually increasing HA and BSA
concentrations. Concentrated high-molecular HA network led to stiffer hydrogel
with lower cluster size and swelling capacity. Medium and oxygen diffusion
capability of all hydrogels showed they are suitable for 3D cell cultures.
Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on
hydrogels were compared with cells on standard cultivation surfaces. Experiments
showed that hydrogels have suitable mechanical and cell adhesion capabilities,
resulting in structural changes of actin filaments. Lastly, applying hydrogel
for a more complex HL-1 cell line revealed improved mechanical and
electrophysiological contractile properties.
Copyright © 2024 Elsevier B.V. All rights reserved.
DOI: 10.1016/j.bioadv.2024.213819
PMID: 38430724 [Indexed for MEDLINE]
Conflict of interest statement: Declaration of competing interest The authors
declare that the research was conducted without any commercial or financial
relationships that could be construed as a potential conflict of interest.