Analysis of biogenic amines by solid-phase microextraction and high-performance liquid chromatography with electrochemical detection
Journal of Chromatography A. 2000-02-01; 870(1-2): 395-403
DOI: 10.1016/S0021-9673(99)01077-8
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Auger J(1), Boulay R, Jaillais B, Delion-Vancassel S.
Author information:
(1)Institut de Recherche sur la Biologie de l’Insecte, Université François
Rabelais, Tours, France.
We investigated the new solid-phase microextraction method by high-performance
liquid chromatography with electrochemical detection for the analysis of
biogenic amines. The Carbowax-Templated Resin 50 microm (purple) fibre coating
offers good performances for dopamine and serotonin separation, i.e., good
selectivity and high sensibility (0.1 microg l(-1)). We also tested this fibre
for biogenic amines quantification of rat striatum. The coating seems to be
selective towards the amines and has low affinity for the metabolites, allowing
a good separation and preventing drawbacks from the biological matrix. These
first results obtained using this original separation method offer large
perspectives of application to many biological samples.