DHA enhances the noradrenaline release by SH-SY5Y cells
Neurochemistry International. 2010-01-01; 56(1): 94-100
DOI: 10.1016/j.neuint.2009.09.006
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Mathieu G(1), Denis S, Langelier B, Denis I, Lavialle M, Vancassel S.
Author information:
(1)INRA, Unité de Nutrition et Régulation Lipidiques des Fonctions Cérébrales
(NuRéLiCe), UR909, Domaine de Vilvert, Jouy en Josas, France.
Erratum in
Neurochem Int. 2010 Nov;57(6):690. Géraldine, Mathieu [corrected to Mathieu,
Géraldine]; Stéphanie, Denis [corrected to Denis, Stéphanie]; Bénédicte,
Langelier [corrected to Langelier, Bénédicte]; Isabelle, Denis [corrected to
Denis, Isabelle]; Monique, Lavialle [corrected to Lavial.
Polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA) and
arachidonic acid (AA), are the main components of the phospholipids, in cerebral
membranes. A dietary-induced cerebral DHA deficit results in altered behaviour
and neurotransmission in rodents. To determine whether PUFA were acting on the
neurotransmitter release machinery, we measured the release of
[(3)H]-noradrenaline (NA) from SH-SY5Y neuroblastoma cells with modified PUFA
membrane contents and from cells incubated with medium containing high DHA or
AA. The membranes of cells incubated with 70 microM DHA for 3 days had 7.6-times
more DHA in their ethanolamine glycerophospholipids, while the membranes of
cells incubated with AA had 40% less. Incorporation of DHA enhanced basal
[(3)H]-NA release (25%, p