Venue: Centre Broca
Cedars-Sinai Health Sciences University,
L.A., USA
Invited by Audrey Dufau (IINS)
Title
Synaptic vesicles that store monoamines and glutamate differ in protein composition and functional properties
Abstract
Neuromodulators like monoamines assure essential brain functions but many open questions remain about their mechanism of release. While recent work has advanced our knowledge of active zones in dopamine axons, release machinery within the synaptic vesicle (SV) remains poorly understood. In our recent work, we addressed differences between the release of monoamines and classical synaptic transmitters by comparing the composition of SVs that contain the vesicular monoamine transporter 2 (VMAT2) versus vesicular glutamate transporter 2 (VGLUT2). Complementing previous work showing that these SV populations differ in frequency dependence, recycling kinetics and biogenesis, we now find differences in abundance and isoform expression of many SV protein families. Validation in primary neurons and brain tissue confirms these findings. Functional analysis shows that loss of differentially expressed proteins has a distinct impact on the recycling of VGLUT2 versus VMAT2 vesicles even within the same neuronal population. These findings provide insights into the molecular diversity of SVs and mechanisms of dopamine release.
Selected publications
https://pubmed.ncbi.nlm.nih.gov/40661501/
https://pubmed.ncbi.nlm.nih.gov/31003725/
https://pubmed.ncbi.nlm.nih.gov/37812725/
https://pubmed.ncbi.nlm.nih.gov/39034608/
Meet the speaker!
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PhD seminars are organized by the NBA, Bordeaux Neurocampus, and the Bordeaux Neurocampus Graduate Program.
