Central mechanisms of pain sensitization
Mécanisme central de la sensibilisation à la douleur
The aim of our project is to elucidate basic mechanisms responsible for cellular and network dysfunctions in rodent models of chronic inflammatory and neuropathic pain. We are particularly interested in the overlapping circuits that underlie both chronic pain and associated comorbidities, e.g. anxiety, ADHD, neurodegenerative diseases. Our hypothesis is that these comorbidities play a crucial role in maintaining chronic pain states by disrupting calcium-dependent sensory integration in the dorsal horn of the spinal cord through the modulation of brain to spinal cord descending pathways. Our focus is on peptidergic, serotoninergic, and miRNA-based mechanisms that influence descending controls and their spinal effects.
To address this question, we develop innovative methodologies to:
- modulate and assess pain behaviour (conditional transgenesis, local infusion of pharmacological drugs and behavioural testing),
- record spinal neurons (in vivo extracellular or patch-clamp recording),
- characterize spinal neurons (Patch-Seq, exosome purification and transcriptomic analysis, high resolution 3D neuronal reconstruction),
- modulate brain to spinal cord pathways (optogenetics).
Université de Bordeaux
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