Ultrastructure neuronale et morphogénèse Archives - Bordeaux Neurocampus

Organ_izing the cells

Vincent Studer — Wed, 23 Dec 2020 10:36:20 +0000

3D cell culture i.e. the art of organ-izing cells by cultivating them in configurations that more closely mimic the in-vivo environment is widely considered as the main route towards more physiologically relevant in vitro models. Yet providing simple, large scale and standardized solutions remains a key challenge for the widespread adoption of such cell-based models in laboratories and later in the pharmaceutical industry. Pioneers in the field of 3D brain cultures undoubtedly demonstrated the huge potential of such models in neuroscience. They also point out that novel tools and materials are required to advance their maturation (complexity) and scalability. This…

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Planar polarity and plasticity

Nathalie Sans — Thu, 07 Jun 2018 16:10:38 +0000

Our group is interested in elucidating the role of the mammalian planar cell polarity (PCP) in the developing and adult nervous system. We study how defects in PCP genes cause sociability impairements and memory imbalances, typical of Autism spectrum disorder. To this goal, we explore the impact of PCP proteins (i.e. Vangl2 and Scrib1) on synaptic functions and characterise the PCP protein complexes and protein interactions. In parallel, we use the inner ear as a model to address the role and mechanisms of PCP signalling and identify novel molecular actors of PCP pathway. Innovative techniques: primary cultures of brain slices…

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Purinergic mediated neuroinflammation and brain disorders

Boué-Grabot Eric — Thu, 24 Sep 2020 11:37:36 +0000

Neurological disorders rely on alterations of the central or peripheral nervous system that are caused by impairments of neuronal transmission or more global perturbations of local circuits that implicate glial cells. The scientific goal of our team is to understand the contribution of neuronal versus inflammatory signaling in brain disorders including amyotrophic lateral sclerosis (ALS), attention deficit/hyperactivity disorders (ADHD), chronic pain, and anxiety-related disorders. Our objectives are to determine (i) the contribution of purinergic signaling as a dual system, and (ii) the modulatory function of peptidergic signaling, both capable to regulate neuronal synaptic activation and microglial neuro-inflammatory reaction as well…

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Spatio-temporal and mechanical control of motile structures

Giannone Gregory — Thu, 07 Jun 2018 16:10:38 +0000

Our goal is to decipher at the molecular level the spatiotemporal and mechanical mechanisms which control the architecture and dynamics of motile structures. First in the context of cell migration by studying integrin-based adhesion sites and the actin-based lamellipodium. Second in the context of neuronal structural plasticity by studying the actin cytoskeleton in axons and dendritic spines. Exploration of these new dimensions requires an innovative and multidisciplinary approach combining cell biology, biophysics, biomechanics and advanced optical microscopy techniques including super-resolution microscopy, single protein tracking and quantitative image analysis. Innovative techniques: single molecule localization microscopy (PALM, STORM, uPAINT), super-resolution microscopy (RESOLFT),…

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