Complexes moléculaires de la synapse Archives - Bordeaux Neurocampus

Developmental Brain Physiology and Pathology

Groc Laurent — Thu, 07 Jun 2018 16:10:38 +0000

A great challenge for our comprehension of brain development is to identify how biological signals (glutamate, monoamine, immune system, hormones) control the maturation of neuronal connections and brain circuit assemblies in healthy conditions as well as in psychotic disorders. In particular, we showed that the adaptation of developing excitatory synapses mainly depend on the dynamics of surface postsynaptic receptor, including the NMDA and AMPA receptor. Our team will combine cutting edge high resolution imaging and classical electrophysiological approaches to detect and decrypt the impact of auto-antibodies from psychotic patients. Innovative techniques: single-molecule imaging, receptor trafficking, ex vivo and in vivo…

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Dynamic organization and function of synapses

Daniel Choquet — Thu, 07 Jun 2018 16:10:22 +0000

Our team pursues a transdisciplinary approach, to study the interplay between the dynamics of the molecular components and the synaptic transmission. Based on advanced imaging techniques, we study AMPA receptors and its molecular partners. We obtained breakthrough data on nano-scale organization, dynamics and interaction of synaptic proteins and membrane trafficking, Our efforts are focused on the understanding of the synaptic components dynamics involved in higher cognitive functions and pathologies, such as Alzheimer disease. Innovative techniques | single particle tracking PALM, STORM, STED, live super resolution imaging, Patch-clamp

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Mechanisms of Adaptive Processes in brain circuits (MAP)

Mathieu Letellier — Tue, 09 Jan 2024 16:37:10 +0000

The brain's ability to constantly adapt its organization to ever-changing stimuli is termed "plasticity" and is a prominent feature of learning and memory in adults, as well as the assembly of neuronal circuits during critical developmental periods. Connections that convey relevant information and are the most active become stronger and stabilize, while less active ones weaken and eventually get eliminated, optimizing the storage and processing of information in the brain. Our research aims to examine the molecular and cellular mechanisms underlying these adaptive processes. We employ a multidisciplinary approach, including single-cell manipulation, electrophysiology, optogenetics, single-cell RNA sequencing, and high-resolution imaging,…

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Membrane traffic at synapses (MemTraS)

David Perrais — Fri, 03 Apr 2020 10:38:20 +0000

Our goal in the team is to study the mechanisms regulating synapse function, focusing on membrane trafficking events in normal brain physiology or in the course of disease. We are interested mainly in the presynaptic element filled with synaptic vesicles fusing to release neurotransmitter molecules as well as the post-synaptic side where post-synaptic receptors are going through cycles of endocytosis and recycling, which is essential for synaptic transmission and plasticity. Finally, we are not only interested in canonical synapses, such as cortical glutamatergic synapses, but also in rare and much less understood synapse populations such as neuromodulatory dopamine synapses. To…

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Organ_izing the cells

Vincent Studer — Wed, 23 Dec 2020 10:36:20 +0000

3D cell culture i.e. the art of organ-izing cells by cultivating them in configurations that more closely mimic the in-vivo environment is widely considered as the main route towards more physiologically relevant in vitro models. Yet providing simple, large scale and standardized solutions remains a key challenge for the widespread adoption of such cell-based models in laboratories and later in the pharmaceutical industry. Pioneers in the field of 3D brain cultures undoubtedly demonstrated the huge potential of such models in neuroscience. They also point out that novel tools and materials are required to advance their maturation (complexity) and scalability. This…

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Planar polarity and plasticity

Nathalie Sans — Thu, 07 Jun 2018 16:10:38 +0000

Our group is interested in elucidating the role of the mammalian planar cell polarity (PCP) in the developing and adult nervous system. We study how defects in PCP genes cause sociability impairements and memory imbalances, typical of Autism spectrum disorder. To this goal, we explore the impact of PCP proteins (i.e. Vangl2 and Scrib1) on synaptic functions and characterise the PCP protein complexes and protein interactions. In parallel, we use the inner ear as a model to address the role and mechanisms of PCP signalling and identify novel molecular actors of PCP pathway. Innovative techniques: primary cultures of brain slices…

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Spatio-temporal and mechanical control of motile structures

Giannone Gregory — Thu, 07 Jun 2018 16:10:38 +0000

Our goal is to decipher at the molecular level the spatiotemporal and mechanical mechanisms which control the architecture and dynamics of motile structures. First in the context of cell migration by studying integrin-based adhesion sites and the actin-based lamellipodium. Second in the context of neuronal structural plasticity by studying the actin cytoskeleton in axons and dendritic spines. Exploration of these new dimensions requires an innovative and multidisciplinary approach combining cell biology, biophysics, biomechanics and advanced optical microscopy techniques including super-resolution microscopy, single protein tracking and quantitative image analysis. Innovative techniques: single molecule localization microscopy (PALM, STORM, uPAINT), super-resolution microscopy (RESOLFT),…

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Synapses and neural circuits in behaviour

Mario Carta — Thu, 07 Jun 2018 16:10:38 +0000

The research carried out in our group ambitions to link cell biological mechanisms of proteins and lipids involved in glutamatergic synaptic function and dysfunction. Much of our work has focused on the roles of kainate receptors, a subject in which the group is internationally recognised. We elucidated new biophysical and pharmacological properties of kainate receptors in the developing hippocampal circuits and in temporal lobe epilepsy. Our group has also broken new ground with highly original work on the role of prostaglandins and other lipids in synaptic plasticity. Great efforts are made to develop new methods for investigating the connectivity and…

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