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X-WR-CALNAME:Bordeaux Neurocampus
X-ORIGINAL-URL:https://www.bordeaux-neurocampus.fr
X-WR-CALDESC:Évènements pour Bordeaux Neurocampus
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DTSTART;TZID=Europe/Paris:20211001T140000
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DTSTAMP:20260403T220624
CREATED:20210526T085549Z
LAST-MODIFIED:20210908T103956Z
UID:135306-1633096800-1633096800@www.bordeaux-neurocampus.fr
SUMMARY:Monthly conference (PhD seminar) - Vladan Lucic
DESCRIPTION:\n	\n		\n			Centre Broca Nouvelle-Aquitaine \n\n\n		\n	\n\n	\n		\n			Title : Architecture of trans-synaptic assemblies at the single nanometer scale. \nNeurotransmitter release is mediated by a complex and highly regulated molecular machinery. We imaged isolated synapses by cryo-electron tomography (cryo-ET)\, a method that utilizes faithful sample preservation and direct 3D imaging of fully hydrated\, unlabeled biological material at 2-4 nanometer resolution. The computational procedures for template-free detection and classification in cryo-tomograms that we developed allowed us to quantitatively analyze the spatial organization and inter-relation of synaptic complexes. We showed that tripartite trans-synaptic assemblies (subcolumns) link synaptic vesicles to postsynaptic ionotropic glutamate receptors\, and established that a particular displacement between the interacting complexes characterizes subcolumns. Furthermore\, we obtained de novo average structures of ionotropic glutamate receptors in their physiological composition\, embedded in plasma membrane. In a separate set experiments\, we found that Munc13 and SNAP25\, proteins known to be essential for synaptic vesicle priming for release and fusion\, control the location of synaptic vesicles at a single nanometer precision by tethering them to the plasma membrane\, resulting in two stable primed vesicle states. Furthermore\, these two proteins\, together with protein kinase C\, control the transition between the two primed states by coordinated reorganization of both tethers and molecular bridges (connectors) that interlink vesicles. Together\, our data supports the hypothesis that synaptic transmission is carried by precisely aligned trans-synaptic units that are organized in large molecular assemblies of variable architecture. \nPublications\nMartinez-Sanchez wt al.\, 2021. Trans-synaptic assemblies link synaptic vesicles and neuroreceptors. Science advances\n7: eabe6204. \nMartinez-Sanchez et al.\, 2020. Template-free detection and classification of membrane-\nbound complexes in cryo-electron tomograms. Nature methods 17:209–216. \nZuber\, B.\, and V. Lucic\, 2019. Molecular architecture of the presynaptic terminal. Current opinion\nin structural biology 54:129–138. \n\nOrganized by Bordeaux Neurocampus\, NBA and Bordeaux Neurocampus Graduate Program \n\n		\n	\n\n	\n		\n			 \nVladan Lucic\nProject Group Leader\nResearch Department Molecular Structural Biology\nMax Planck Institute of Biochemistry\nMartinsried\, Munich\, Germany \nhttps://www.biochem.mpg.de/7416282/dr-vladan-lucic \n\n		\n	\n»Share\n\n	\n		\n			Ph.D. students are invited to a networking event with the guest speaker around some pizzas. \nDeadline to register: 48h prior the event\nRegistration form \nNote: According to the legislation in vigour\, you may be requested to show your sanitary pass to attend this event \n  \n\n		\n	\n\n
URL:https://www.bordeaux-neurocampus.fr/event/phd-seminar-october-2021/
CATEGORIES:A la une,Conférences mensuelles,Pour les scientifiques
ATTACH;FMTTYPE=image/jpeg:https://www.bordeaux-neurocampus.fr/wp-content/uploads/2021/05/lucic-vladan-vign.jpg
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