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X-WR-CALNAME:Bordeaux Neurocampus
X-ORIGINAL-URL:https://www.bordeaux-neurocampus.fr
X-WR-CALDESC:Évènements pour Bordeaux Neurocampus
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DTSTART:20190331T010000
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DTSTART:20191027T010000
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DTSTART;TZID=Europe/Paris:20190906T113000
DTEND;TZID=Europe/Paris:20190906T113000
DTSTAMP:20260409T123048
CREATED:20190226T171048Z
LAST-MODIFIED:20200316T133444Z
UID:103731-1567769400-1567769400@www.bordeaux-neurocampus.fr
SUMMARY:Conférence mensuelle (PhD seminar series) - Laurent Bourdieu
DESCRIPTION:Lieu : Centre Broca Nouvelle-Aquitaine \n\nLaurent Bourdieu\nInstitut de Biologie de l’école normale supérieure – Paris\nhttp://www.biologie.ens.fr/neuroctx/ \nInvité par Bordeaux Neurocampus et la NBA \n\nAbstract\n2-photon microscopy allows recordings structural and functional information deep in scattering biological tissues with cellular resolution. However\, standard light steering techniques are slow\, providing frame rates of a few tens of Hz\, and are limited to a plane of interest. We recently developed a new type of 2-photon microscope able to sample neuronal activity from 3D cortical networks in head-fixed animals at kilohertz rate. The microscope operates in a non-imaging mode\, where individual laser pulses are spatially modulated with tilt and defocus to random-address individual cell bodies within a 400x400x400 µm3 volume. Single pulse modulation is achieved by a large aperture\, two-axis acousto-optic deflectors (AODs) synchronized to a 40 kHz regenerative laser amplifier. Individual pulses are further modulated in phase and amplitude to pattern the beam into a square array of up to 25 focal spots targeting the bodies of individual cells\, interleaved with a pattern of 10 spots targeting the neuropil. This spatial patterning helped (1) to raise the single pulse photon yield; (2) to dampen the occurrence of artefacts induced by locomotion and (3) to suppress contamination of somatic signal by surrounding neuropil activity. We validated the microscope by recording single unit activity from neurons in primary visual cortex of layer 2/3 and 5A while the animal was free to move on a treadmill. In agreement with published data we find a significantly higher level of spontaneous activity of layer 5 cells\, including a higher incidence of large amplitude burst discharge\, while layer 2/3 cells were more sensitive to the orientation of a moving contrast grating presented as a visual stimulus. Finally\, the layered organization of the evoked activity could be revealed directly by the analysis of neuron pairwise correlations. \nSélection de publications\n\nFast confocal fluorescence imaging in freely behaving miceClara Dussaux\, Vivien Szabo\, Yan Chastagnier\, Jozsua Fodor\, Jean-François Léger\, Laurent Bourdieu\, Julie Perroy\, Cathie Ventalon\, 2018 \, Scientific Reports 8\, Article number: 16262 \nFocusing ligth through dynamical samples using fast continuous wavefront optimizationB. Blochet\, L. Bourdieu\, S. Gigan\, Optics Letters\, 2017\, 42:4994-4997. \nA radial map of multi-whisker correlation selectivity in the rat barrel cortexL. Estebanez\, J. Bertherat\, D. E. Shulz\, L. Bourdieu\, J.-F. Léger Nat Commun. 2016;7:13528. doi: 10.1038/ncomms13528. \nFast spatial beam shaping by acousto-optic diffraction for 3D non-linear microscopy.W. Akemann\, J.-F. Léger\, C. Ventalon\, B. Mathieu\, S. Dieudonné\, L. Bourdieu Opt. Exp. 2015(22); 23:28191-28205 \nCharacterization of the angular memory effect of scattered light in biological tissues.S. Schott\, J. Bertolotti\, J.F. Léger\, L. Bourdieu\, S. Gigan\, Opt. Exp. 2015(10); 23:13505-13516 \nSpatially Selective Holographic Photoactivation and Functional Fluorescence Imaging in Freely Behaving Mice with a Fiberscope.V. Szabo*\, C. Ventalon*\, V. De Sars\, J. Bradley\, V. Emiliani\,Neuron. 2014; 84(6):1157–1169 \nLate emergence of the vibrissa direction selectivity map in the rat barrel cortex.Kremer Y\, Léger JF\, Goodman D\, Brette R\, Bourdieu L.J Neurosci. 2011; 31(29):10689-700. \n\n
URL:https://www.bordeaux-neurocampus.fr/event/conference-mensuelle/
CATEGORIES:A la une,Conférences mensuelles,Pour les scientifiques
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