{"id":193748,"date":"2026-02-05T11:26:10","date_gmt":"2026-02-05T10:26:10","guid":{"rendered":"https:\/\/www.bordeaux-neurocampus.fr\/?p=193748"},"modified":"2026-02-05T12:24:46","modified_gmt":"2026-02-05T11:24:46","slug":"affinity-guided-labeling-reveals-p2x7-nanoscale-membrane-redistribution-during-bv2-microglial-activation","status":"publish","type":"post","link":"https:\/\/www.bordeaux-neurocampus.fr\/en\/affinity-guided-labeling-reveals-p2x7-nanoscale-membrane-redistribution-during-bv2-microglial-activation\/","title":{"rendered":"Affinity-guided labeling reveals P2X7 nanoscale membrane redistribution during BV2 microglial\u00a0activation"},"content":{"rendered":"<div class=\"introduction\">\n<div class=\"clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item\">\n<div class=\"tex2jax_process\">\n<div class=\"introduction\">\n<div class=\"clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item\">\n<div class=\"tex2jax_process\">\n<div class=\"introduction\">\n<div class=\"clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item\">\n<div class=\"tex2jax_process\">\n<p class=\"text-align-justify\">Pauline Belzanne and Eric Hosy (IINS) collaborated to an article in eLife.<\/p>\n<h3>Abstract<\/h3>\n<p class=\"text-align-justify\">ATP-gated purinergic P2X7 receptors are crucial ion channels involved in inflammation. They sense abnormal ATP release during stress or injury and are considered promising clinical targets for therapeutic intervention. However, despite their predominant expression in immune cells such as microglia, there is limited information on P2X7 membrane expression and regulation during inflammation at the single-molecule level, necessitating new labeling approaches to visualize P2X7 in native cells. Here, we present X7-uP, an unbiased, affinity-guided P2X7 chemical labeling reagent that selectively and covalently biotinylates endogenous P2X7 in BV2 cells, a murine microglial cell line, allowing subsequent labeling with streptavidin-Alexa 647 tailored for super-resolution imaging. We uncovered a nanoscale microglial P2X7 redistribution mechanism where evenly spaced individual receptors in quiescent cells undergo upregulation and clustering in response to the pro-inflammatory agent lipopolysaccharide and ATP, leading to synergistic interleukin-1\u03b2 release. Our method thus offers a new approach to revealing endogenous P2X7 expression at the single-molecule level.<\/p>\n<p><strong><a href=\"https:\/\/www.inc.cnrs.fr\/fr\/cnrsinfo\/observer-les-recepteurs-de-linflammation-au-nanometre-pres-grace-la-chimie\">See the CNRS chimie news (in french)<\/a><\/strong><\/p>\n<h3>Reference<\/h3>\n<p><a href=\"https:\/\/doi.org\/10.7554\/eLife.106096.3\"><strong>Affinity-guided labeling reveals P2X7 nanoscale membrane redistribution during BV2 microglial\u00a0activation<\/strong><\/a><br \/>\nBenoit Arnould, Adeline Martz, <strong>Pauline Belzanne<\/strong>, Francisco Andr\u00e9s Peralta, Federico Cevoli, Volodya Hovhannisyan, Yannick Goumon, <strong>Eric Hosy<\/strong>, Alexandre Specht &amp; Thomas Grutter<br \/>\n<em>eLife, <\/em>02 f\u00e9vrier 2026<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Pauline Belzanne and Eric Hosy (IINS) collaborated <\/p>\n","protected":false},"author":357,"featured_media":193749,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[71,770],"tags":[],"class_list":["post-193748","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-highlight-en","category-iins-en"],"_links":{"self":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts\/193748","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/users\/357"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/comments?post=193748"}],"version-history":[{"count":5,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts\/193748\/revisions"}],"predecessor-version":[{"id":193766,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts\/193748\/revisions\/193766"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/media\/193749"}],"wp:attachment":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/media?parent=193748"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/categories?post=193748"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/tags?post=193748"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}