{"id":19290,"date":"2017-02-23T17:17:36","date_gmt":"2017-02-23T16:17:36","guid":{"rendered":"https:\/\/neurodev-ng.u-bordeaux.fr\/d-perrais-m-rosendale-incell-reports\/"},"modified":"2018-03-20T17:54:32","modified_gmt":"2018-03-20T16:54:32","slug":"d-perrais-m-rosendale-incell-reports","status":"publish","type":"post","link":"https:\/\/www.bordeaux-neurocampus.fr\/en\/d-perrais-m-rosendale-incell-reports\/","title":{"rendered":"D.Perrais, M. Rosendale in<em>Cell Reports<\/em>"},"content":{"rendered":"<p>February 23rd, 2017<\/p>\n<p><em>Rosendale M, Julli\u00e9 D, Choquet D, Perrais D. (2017) <a class=\"external\" href=\"http:\/\/www.cell.com\/cell-reports\/abstract\/S2211-1247(17)30166-3\"><strong>Spatial and temporal regulation of receptor endocytosis in neuronal dendrites revealed by imaging of single vesicle formation.<\/strong> <\/a>Cell Reports, Feb 21<br \/>\n<\/em><\/p>\n<p>David Perrais \u00a0\/IINS Bordeaux \/ Nano-scale organization, dynamics of synaptic proteins and membrane trafficking. Morgane Rosendale: Brain Research Institute RIKEN, Japon<\/p>\n<hr \/>\n<p><strong>\u00a0<\/strong><\/p>\n<p>&nbsp;<\/p>\n<figure style=\"width: 591px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.bordeaux-neurocampus.fr\/_contents-images\/ametys-internal%253Asites\/neurosciences\/ametys-internal%253Acontents\/perrais-cell-reports-actualite\/_metadata\/content\/_data\/Rosendale1-Press-release-Figure-2.jpg_443x591\" alt=\"\" width=\"591\" height=\"443\" \/><figcaption class=\"wp-caption-text\">Figure legend: Endocytic event detected with TfR-SEP occurring at time 0 in a spine. Right, merged TfR (pH 7.4) and Homer1c, a PSD marker, with the location of the event (cross).<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p><strong><em><span class=\"image-wrapper frame floatleft\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft\" src=\"https:\/\/www.bordeaux-neurocampus.fr\/_contents-images\/ametys-internal%253Asites\/neurosciences\/ametys-internal%253Acontents\/perrais-cell-reports-actualite\/_metadata\/content\/_data\/Perrais-Rosendale.jpg_160x250\" alt=\"\" width=\"250\" height=\"160\" \/><\/span>Spatial and temporal regulation of receptor endocytosis in neuronal dendrites revealed by imaging of single vesicle formation<\/em><\/strong><\/p>\n<p><em>Endocytosis in neuronal dendrites is known to play a critical role in synaptic transmission and plasticity such as long term depression (LTD). However, the inability to detect endocytosis directly in living neurons has hampered studies of its dynamics and regulation. Here we visualized the formation of individual endocytic vesicles containing pHluorin tagged receptors with high temporal resolution in dendrites of cultured hippocampal neurons. We show that transferrin receptors (TfRs) are constitutively internalized at optically static clathrin coated structures. These structures are slightly enriched near synapses which represent preferential sites for the endocytosis of postsynaptic AMPA-type receptors (AMPARs), but not for non-synaptic TfRs. Moreover, the frequency of AMPAR endocytosis events increases after induction of NMDAR-dependent chemical LTD but the activity of perisynaptic endocytic zones is not differentially regulated. <\/em><\/p>\n<p><em>We conclude that endocytosis is a highly dynamic and stereotyped process which internalizes receptors in precisely located endocytic zones.<br \/>\n<\/em><\/p>\n<figure style=\"width: 700px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.bordeaux-neurocampus.fr\/_contents-images\/ametys-internal%253Asites\/neurosciences\/ametys-internal%253Acontents\/perrais-cell-reports-actualite\/_metadata\/content\/_data\/Perrais-Graphical_Abstract2.jpg_700x700\" alt=\"\" width=\"700\" height=\"700\" \/><figcaption class=\"wp-caption-text\">Map of endocytic events detected in an 8 min recording overlaid on the corresponding Homer1c-RFP image. Blue and pink crosses locate events detected more or less than 300 nm away from a PSD, respectively.<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>February 23rd, 2017 Rosendale M, Julli\u00e9 D, Choquet D, Perrais D. (2017) Spatial and temporal regulation of receptor endocytosis in neuronal dendrites revealed by imaging of single vesicle formation. Cell Reports, Feb 21 David Perrais \u00a0\/IINS Bordeaux \/ Nano-scale organization, dynamics of synaptic proteins and membrane trafficking. Morgane Rosendale: Brain Research Institute RIKEN, Japon \u00a0&hellip;<\/p>\n","protected":false},"author":108,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[71],"tags":[],"class_list":["post-19290","post","type-post","status-publish","format-standard","hentry","category-highlight-en"],"_links":{"self":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts\/19290","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/users\/108"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/comments?post=19290"}],"version-history":[{"count":0,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts\/19290\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/media?parent=19290"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/categories?post=19290"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/tags?post=19290"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}