{"id":155589,"date":"2023-02-16T10:04:34","date_gmt":"2023-02-16T09:04:34","guid":{"rendered":"https:\/\/www.bordeaux-neurocampus.fr\/?p=155589"},"modified":"2023-02-16T10:06:08","modified_gmt":"2023-02-16T09:06:08","slug":"implementation-of-spatial-transcriptomics","status":"publish","type":"post","link":"https:\/\/www.bordeaux-neurocampus.fr\/en\/implementation-of-spatial-transcriptomics\/","title":{"rendered":"Implementation of Spatial Transcriptomics"},"content":{"rendered":"

The Laser Microdissection and Transcriptome facilities (Puma, Neurocentre Magendie) wish to develop Spatial Transcriptomics technology.<\/p>\n

Indeed, Fr\u00e9d\u00e9ric Martins, an engineer who recently arrived in the Transcriptome facility, has already carried out this type of experiment in his previous unit. He is therefore looking for interested users.<\/p>\n

\"\"<\/a>The goal of Spatial Transcriptomics (Visium technology, 10x Genomics) is to capture mRNAs via their poly-A tail and tag them with a spatial barcode. This barcode then allows them to be analyzed and have their spatial origin identified.<\/p>\n

The analysis of the transcriptome is done with a spatial resolution of 50\u00b5m and allows us to:
\n– evaluate the differential expression within different tissue areas (for example between the hippocampus, the thalamus and the hypothalamus)
\n– know the highest gene expression by area
\n– generate a list of co-expressed genes
\n– represent gene-to-gene mapping<\/p>\n

This technology requires no investment in terms of equipment and allows us to work on frozen tissue sections (from all organisms) or FFPE (human and mouse) with sizes of 6.5mm x 6.5mm, i.e. the equivalent of a mouse hemisphere. This technology is therefore perfectly suited to the study of the brain.<\/p>\n

Contact<\/h3>\n

PUMA, Neurocentre Magendie<\/p>\n

Fr\u00e9d\u00e9ric Martins
\n05 57 57 36 79
\nfrederic.martins@inserm.fr<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

PUMA, Neurocentre Magendie<\/p>\n","protected":false},"author":108,"featured_media":155603,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[345],"tags":[],"class_list":["post-155589","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-platforms-news"],"_links":{"self":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts\/155589","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/users\/108"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/comments?post=155589"}],"version-history":[{"count":2,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts\/155589\/revisions"}],"predecessor-version":[{"id":155605,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/posts\/155589\/revisions\/155605"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/media\/155603"}],"wp:attachment":[{"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/media?parent=155589"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/categories?post=155589"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bordeaux-neurocampus.fr\/en\/wp-json\/wp\/v2\/tags?post=155589"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}