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X-WR-CALNAME:Bordeaux Neurocampus
X-ORIGINAL-URL:https://www.bordeaux-neurocampus.fr/en/
X-WR-CALDESC:Events for Bordeaux Neurocampus
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DTSTART:20230326T010000
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DTSTART;VALUE=DATE:20231024
DTEND;VALUE=DATE:20231110
DTSTAMP:20260410T160852
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LAST-MODIFIED:20231109T141649Z
UID:152946-1698105600-1699574399@www.bordeaux-neurocampus.fr
SUMMARY:Cajal lectures: Advanced techniques for synapse biology
DESCRIPTION:Venue: CGFB \nFree access \n\nOctober 24 – 9:00am \nCécile Charrier (Institute of Biology\, École Normale Supérieure\, France)\nMolecular mechanisms of synaptic development: insights from a human-specific gene. \nOctober 24 – 11:00am \nBrian Mac Cabe (EPFL\, Lausanne\, Swiss)\nUnknown knowns of Drosophila synapses. \nOctober 26 – 9:00am\n Noa Lipstein (LeibnizFMP\, Germany)\nSynaptic transmission in health and disease. \nOctober 26 – 11:00am\n Julie Perroy (IGF\, University of Montpellier\, France)\nMolecular dynamics at glutamatergic synapses and beyond. \nOctober 28 – 9:00am \nJosef Kittler (University College London\, UK)\nMolecular mechanism of inhibitory synapse formation and plasticity. \nOctober 30 – 9:00am \nDaniel Choquet (CNRS/University of Bordeaux\, France)\nNanoscale synapse organization and function. \nNovember 2 – 9:00am \nMarina Mikhaylova (Humboldt University \, Germany)\nCalcium and synaptic heterogeneity. \nNovember 3 – 9:00am\nRosa Paolicelli (University of Lausanne\, Swiss)\nMicroglia: key players in synapse remodeling in the healthy and diseased brain. \nNovember 3 – 11:00am \nAlfredo Kirkwood (Johns Hopkins University\, USA)\nPrinciples of Hebbian\, Pavlovian and Homeostatic synaptic plasticity. \nNovember 6 – 9:00am \nJuan Burrone (King’s College London\, UK)\nThe emergence and plasticity of inhibitory synapses: from dendrites to the axon initial segment. \nNovember 6 – 11:00am \nAxion BioSystems : Presentation \nNovember 7 – 9:00am \nNael Nadif Kasri (Radboud University Medical Center\, Netherlands)\nLeveraging spontaneous activity in human neuronal stem cell-derived neurons to model neurodevelopmental disorders. \nNovember 9 – 9:00am \nChristian Lohmann (Netherlands Institute for Neuroscience\, Netherlands)\nImaging synapse development. \nNovember 9 – 11:00am \nJulijana Gjorgjieva (Max Planck Institute for Brain Research\, Germany)\nEmergence of organization and computations at the subcellular and cellular scales. \nCourse directors\nAna Luisa Carvalho – Coimbra University\, Portugal \nMathieu Letellier – Bordeaux University\, France \nHey-Kyoung Lee – John Hopkins University.\, US \nAbout the course\nAdvanced techniques for synapse biology – CAJAL (cajal-training.org) \n
URL:https://www.bordeaux-neurocampus.fr/en/event/advanced-techniques-for-synapse-biology/
CATEGORIES:Cajal Lectures,For scientists
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DTSTART;TZID=Europe/Paris:20231103T113000
DTEND;TZID=Europe/Paris:20231103T113000
DTSTAMP:20260410T160852
CREATED:20230505T103506Z
LAST-MODIFIED:20231025T073258Z
UID:159231-1699011000-1699011000@www.bordeaux-neurocampus.fr
SUMMARY:Monthly conference (PhD seminar) - Claudia Verderio
DESCRIPTION:Venue: Centre Broca \n\nClaudia Verderio\nInstitute of Neurosciences\, Milan\, Italy \nInvited by Rebecca Hekking (Oliet’s team – Magendie) \nTitle\nMicroglial EVs travelling at the neuron surface: implication in synaptic pruning and abeta-related  synaptic dysfunction \nAbstract\nUsing optical tweezers combined to time-lapse imaging\, we studied EV-neuron interaction dynamics in vitro coming to the unexpected observation that a large fraction of glial EVs move along the surface of hippocampal neurons\, scanning actin protrusions (D’Arrigo et al.\, JEV 2021; Gabrielli et al.\, Brain 2022). In fully differentiated cultures\, EVs frequently stop moving at synaptic sites on dendrites\, while the fraction of moving EVs and average EV motion are elevated along axons. These data\, together with previous evidence showing that microglial EVs carry multiple signals implicated in synaptic pruning (PS\, complement factors)\, prompted us to investigate whether EVs may deliver molecules guiding microglia-mediated synaptic removal on dendrites and exploit axonal projections as highways to reach target neurons to spread pathological signals across the synapse. In my talk I go in deep into our research on these topics. \nThe hypothesis that EVs may use axons to move between synaptically connected neurons and spread their cargo was tested in a neurodegenerative context. According to our data\, the injection of large microglial EVs carrying Aβ species (Aβ-EVs) induces and propagates amyloid-related synaptic deficits among synaptically connected regions in a circuit primarily affected in Alzheimer’s disease (AD)\, i.e. the entorhinal-hippocampal circuit\, leading to progressive memory impairment and persistent network alterations both at hippocampal and cortical level (Gabrielli et. al\, Brain\, 2022; Falcicchia et al\, Brain Comm\, 2023). When the motility of Aβ-EVs is inhibited\, there is no synaptic dysfunction propagation nor network stability impairment. These data unveil motion of large Aβ-EVs at the neuron surface as a new mechanism contributing to the diffusion of Aβ-related pathology in AD. \nTo explore EV involvement in synaptic pruning\, we co-cultured neurons with wild type (wt) or mutant (C9orf72 knock out) microglia\, which produce more EVs and complement factors compared to wt\, and analyzed synaptic density\, finding that mutant microglia enhance engulfment of pre-synaptic material. Interestingly\, pretreatment of mutant microglia with GW4869\, an inhibitor of EV biogenesis\, restored normal pre-synaptic density\, while supplementation of microglial EVs to neurons decreased pre-synaptic density and favored engulfment of synaptic material/synaptosomes by microglia\, revealing that EVs promote synaptic pruning. Immunofluorescence analysis of C9orf72 ko hippocampi at P17 (peak synaptic pruning) showed lower density of Vglut+ pre-synapses and revealed higher C1q staining in CA1 area compared to wt\, linking larger EV production to enhanced C1q deposition and excessive pruning. \nOur studies shed light on the possible roles of microglial EVs in synaptic alterations in neurodevelopmental and neurodegenerative disorders. \nPublications\nD’Arrigo G\, Gabrielli M\, Scaroni F\, Swuec3 P\, Amin L\, Pegoraro A\, Adinolfi E\, Di Virgilio F\, Cojoc D\, Legname G\, Verderio C (2021) Astrocytes-derived extracellular vesicles in motion at the neuron surface: involvement of the prion protein. JEV\, Jul;10(9):e12114. \nGabrielli M\, Prada I\, Joshi P\, Falcicchia C\, D’Arrigo G\, Rutigliano G\, Battocchio E\, Zenatelli R\, Tozzi F\, Radeghieri A\, Arancio O\, Origlia N* and Verderio C* (2022) Microglial large extracellular vesicles propagate early synaptic dysfunction in Alzheimer’s disease. Brain\, 45(8):2849-2868. \nFalcicchia C\, Tozzi F\, Gabrielli M\, Amoretti S\, Masini G\, Nardi G\, Guglielmo S\, Ratto GM\, Arancio O\, Verderio C*\, Origlia N* (2023)(*co-corresponding author). Microglial extracellular vesicles induce Alzheimer’s disease-related cortico-hippocampal network dysfunction. Brain Commun. May 31;5(3): fcad170. doi: 10.1093/braincomms/fcad170. \n\nPhD seminars are organized by the NBA\, Bordeaux Neurocampus\, and the Bordeaux Neurocampus Graduate Program \n
URL:https://www.bordeaux-neurocampus.fr/en/event/monthly-conference-phd-seminar-november2023/
CATEGORIES:For scientists,home-event,Monthly conferences
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