BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Bordeaux Neurocampus - ECPv4.9.10//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-WR-CALNAME:Bordeaux Neurocampus
X-ORIGINAL-URL:https://www.bordeaux-neurocampus.fr/en/
X-WR-CALDESC:Events for Bordeaux Neurocampus
BEGIN:VTIMEZONE
TZID:Europe/Paris
BEGIN:DAYLIGHT
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
TZNAME:CEST
DTSTART:20250330T010000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:+0200
TZOFFSETTO:+0100
TZNAME:CET
DTSTART:20251026T010000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;VALUE=DATE:20250919
DTEND;VALUE=DATE:20250920
DTSTAMP:20260525T152513
CREATED:20250114T165014Z
LAST-MODIFIED:20250919T144704Z
UID:179865-1758240000-1758326399@www.bordeaux-neurocampus.fr
SUMMARY:Synapse and Network Day 2025
DESCRIPTION:The 22nd edition of the Synapse and Network Day will take place at the Centre Broca on Friday 19 September from 9:30. \nRegistrations for lunch are closed. \nPlease note that Shujia Zhu’s talk has been moved to Thursday 18 September at 2pm (venue: CARF) \nGuest\n\nPhilippe Isope (INCI\, université de Strasbourg)\nHow synaptic connectivity maps encode internal models in the cerebellar cortex?\n\nProgram\n9:30-10:30 – Philippe Isope (INCI\, Université de Strasbourg)\nHow synaptic connectivity maps encode internal models in the cerebellar cortex? \n10:30-10:50 – Silvia Sposini (IINS)\nImaging the dynamics of neuromodulator receptor endocytosis \n10:50-11:10 – Juan Garcia Ruiz (Neurocentre Magendie)\nThe role of glucose and lactate to sustain basal synaptic transmission and excitability \n11:10-11:30 – Break \n11:30-12:00 – Romuald Nargeot (INCIA)\nOrganelle calcium-derived voltage oscillations in a decisional network contribute to memory of a compulsive-like behavior in Aplysia \n12:00-12:20 – Simon Lecomte (IINS)\nImpact of FMRP deletion on presynaptic mechanisms at mf-CA3 synapse. \n12:20-12:40 – Carmen Guerrero-Marquez (IMN)\nCortical Contributions to Basal Ganglia-Thalamocortical Loop Dynamics in the Zebra Finch Song System \n12:40-14:20 – Lunch break \n14:20-14:50 – Sandrine Pouvreau (Neurocentre Magendie)\nMitochondrial-cytosolic calcium crosstalk in astrocytes: how do CB1 receptors shape the dialogue? \n14:50-15:10 – Legeolas Velez (IINS)\nExcitatory synaptic plasticity onto inhibitory interneurons during learning. \n15:10-15:40 – Julien Courtin  (Neurocentre Magendie)\nMemory transfer within amygdala circuits \n15:40-16:00 – Break \n16:00-16:20 – Jakob Scharnholz (IMN)\nIs there a link between protein aggregation and dopamine physiology failure in the context of Parkinson disease? \n16:20-16:40 – Brice de La Crompe (INCIA)\nUsing open-resources to explore the role of the premotor cortex in flexible behaviour. \n16:40-17:10 – Julien Dupuis (IINS)\nKetamine alleviates NMDA receptor hypofunction through synaptic trapping \n17:10 – Concluding remarks \nOrganizing committee\n\nElena Avignone (Neurocentre Magendie)\nMario Carta (IINS)\nFrédéric Lanore (IINS)\nMuriel Thoby Brisson (INCIA)\n\n
URL:https://www.bordeaux-neurocampus.fr/en/event/network-and-synapse-day-2025/
CATEGORIES:For scientists,IINS,iNCIA,Magendie,Symposium
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Paris:20250919T093000
DTEND;TZID=Europe/Paris:20250919T113000
DTSTAMP:20260525T152513
CREATED:20250910T163131Z
LAST-MODIFIED:20250910T163131Z
UID:187820-1758274200-1758281400@www.bordeaux-neurocampus.fr
SUMMARY:Seminars - Christophe Zimmer and Thomas Walter
DESCRIPTION:CARF Building Amphitheater \nIn the context of Christer Lohk’s PhD defense \n\n9:30 AM – Christophe Zimmer\nRudolf Virchow Zentrum\, Würzburg\, Germany\nAI for Microbiology and Epidemiology \n10:30 AM – Thomas Walter\nInstitut Curie\, Paris\, France\nComputer Vision for Spatial Transcriptomics \nSpatial Transcriptomics (ST) is a technique that maps gene expression to specific locations within a tissue\, thereby combining spatial information with transcriptomic data. ST enables the study of the spatial organization of cell types and cellular states\, and how these relate to tissue structure and function. ST poses several computational challenges\, including image segmentation\, single-cell deconvolution\, and cross-modality prediction\, where the goal is to infer transcriptomic profiles from morphological phenotypes. \nIn this lecture\, Dr. Walter will present recent developments in these areas. He will discuss methods for predicting molecular signatures from histology images\, as well as work on cell segmentation from image-based spatial transcriptomics data. Finally\, he will introduce an approach for predicting single-cell gene expression from morphological phenotypes\, leveraging paired Hematoxylin & Eosin (H&E) and spatial transcriptomics data. \n
URL:https://www.bordeaux-neurocampus.fr/en/event/seminars-christophe-zimmer-and-thomas-walter/
CATEGORIES:For scientists,home-event,Impromptu seminar
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Paris:20250919T140000
DTEND;TZID=Europe/Paris:20250919T140000
DTSTAMP:20260525T152513
CREATED:20250428T072503Z
LAST-MODIFIED:20250731T080424Z
UID:183455-1758290400-1758290400@www.bordeaux-neurocampus.fr
SUMMARY:Thesis defense - Ænora Letourneur
DESCRIPTION:Venue : CARF \n \nOn zoom: https://u-bordeaux-fr.zoom.us/j/83810704899 \nThèse défendue en français \n\nÆnora Letourneur \nTeam : SynTeam\nIMN \nTitle\nThe chaperone DNAJB6 as a regulator of alpha-synuclein aggregation in neuronal models of induced synucleinopathy \nAbstract\nThe protein α-synuclein self-assembles into amyloid fibrils\, found in the neuronal or glial inclusion observed in synucleinopathies. In vitro studies have characterized the aggregation mechanisms of the protein\, but the processes that the cell can use to modulate this aggregation are still under investigation. We chose to work in models of induced synucleinopathy\, both primary cultures and in adult mice brains. \nWe first look at a panel of molecular chaperones\, which have been shown to be involved in this regulation\, before focusing of DNAJB6. Indeed\, we observed\, with this protein\, a specific relocalization of DNAJB6\, condensed as punctae\, to the neo-formed aggregates. This observation\, combined with the studies showing DNAJB6 to have an affinity for amyloid structures\, made us hypothesize that DNAJB6 had a functional role in the regulation of the induced synucleinopathy. \nIndeed\, functional studies then allowed us to observe an inhibition of the aggregation of α-syn when DNAJB6 is overexpressed\, both in primary cultures and in mice brains. We deduced that this effect was due to a selective interaction of DNAJB6 with the fibrillar α-syn\, and not the monomeric one. Furthermore\, we were able to show that this inhibition of the aggregation was due to the activity of DNAJB6 alone and not\, as we thought in the beginning\, due to its involvement in a system also containing Hsp110 and Hsc70\, for which DNAJB6 is a co-chaperone. Indeed\, the mutated H31Q DNAJB6\, which cannot interact with Hsc70\, showed the same inhibitory effect as the wild-type DNAJB6. We confirmed this point by reconstituting in vitro the system and seeing that\, in contrast with DNAJB1\, DNAJB6 does not induce a disaggregase activity of the system on the α-syn fibrils. \nKey words\nalpha-synuclein ; synucleinopathy ; molecular chaperones ; DNAJB6 ; neurons \nPublication\nDe Giorgi\, F.\, Letourneur\, A.\, Kashyrina\, M.\, Zinghirino F.\, Dovero\, S.\, Dutheil\, N.\, Largitten L.\, Arotçarena\, M.\, Bezard\, E.\, Canron\, M.\, Meissner\, W.\, De Nuccio\, F.\, Ichas\, F.\, 2024. Reconsidering α-Synuclein inclusion pathology in neurons\, mice\, and humans with an antibody sensing NAC engagement during α-Synuclein amyloid conversion. (preprint)\nJury\nDr. Nocca Smet\, Université de Lille – Rapportrice\nDr. Genevaux Pierre\, Université Toulouse 3 – Rapporteur\nDr. Angot Elodie\, ROCHE – Examinatrice\nDr. Baron Thierry\, Anses Lyon – Examinateur\n
URL:https://www.bordeaux-neurocampus.fr/en/event/thesis-defense-aenora-letourneur/
CATEGORIES:IMN,Thesis
END:VEVENT
BEGIN:VEVENT
DTSTART;TZID=Europe/Paris:20250919T143000
DTEND;TZID=Europe/Paris:20250919T143000
DTSTAMP:20260525T152513
CREATED:20250827T115015Z
LAST-MODIFIED:20250911T095506Z
UID:187284-1758292200-1758292200@www.bordeaux-neurocampus.fr
SUMMARY:Thesis defense - Christer Lohk
DESCRIPTION:Venue: IBGC \n\nChrister Lohk\nTeam: Quantitative imaging of the cell \nIINS \nThesis supervised by Jean-Baptiste Sibarita (IINS) & Macha Nikolski (IBGC) \nTitle\n“Artificial intelligence for the characterization of 3D cultures in high content fluorescence microscopy” \nAbstract\nOver the recent years\, 3D cell cultures have become the gold standard in cell biology\, with applications ranging from fundamental research to high-content drug screening and biomedicine. Among them\, organoids oVer a major advantage over traditional two-dimensional cell cultures as they accurately replicate the cellular architecture and function of a tissue of interest. 3D cell cultures have\, therefore\, become irreplaceable as biological models for studying disease mechanisms\, normal tissue development\, and screening drug responses. \nThe main focus of this thesis project was the development of computational tools for the analysis of 3D cell culture images obtained with the light sheet microscopy-based high- content screening platform developed in our lab. This acquisition system employs disposable microfabricated chips\, containing arrays of small pyramidal wells enabling the cultivation and parallel three-dimensional imaging of individual organoids. With this approach\, imaging data can be acquired simultaneously from over a hundred single organoids using two complementary modalities: i) fluorescence single-objective Selective Plane Illumination Microscopy (soSPIM)\, which captures three-dimensional multi-channel image stacks\, and ii) transmission light modality\, allowing for rapid\, label-free acquisition of two-dimensional images of the samples. When used in conjunction with an integrated well detection algorithm\, the image acquisition process can be fully automated and run over extended periods of time. \nThis thesis presents a toolkit of computational workflows for monitoring and quantification of drug-induced changes in spheroid and organoid cell cultures. Our proposed methods include i) a deep learning-based real-time segmentation model to rapidly localize organoid boundaries in transmission light images\, ii) foundation model- based approach for volumetric segmentation of organoids in three-dimensional images\, and iii) a feature extraction pipeline that integrates classical image features with deep representations from vision transformer and variational autoencoder networks to quantify morphological diVerences across conditions. The feature extraction strategy supports both quality control during acquisition workflow and comprehensive toolset for phenotypic profiling of organoids in fluorescence and brightfield images. Combined with the soSPIM-based culturing and imaging capabilities\, these established methods provide a foundation for future large-scale\, fully automated experimental pipelines targeted at predicting the response of 3D cell cultures to chemical and biological treatments. \nKeywords\n3D cell culture\, image processing and analysis\, fluorescence microscopy\, deep learning \nJury\n\nWALTER Thomas\, Directeur de recherche\, Institut Curie\, Examinateur\nZIMMER Christophe\, Professeur\, Rudolf Virchow Center\, University of Würzburg\, Rapporteur\nDESCOMBES Xavier\, Directeur de recherche\, INRIA Sophia Antipolis\, CNRS\, Rapporteur\nSIBARITA Jean-Baptiste\, Chargé de recherche\, Université de Bordeaux\, CNRS\, Directeur de Thèse\nNIKOLSKI Macha\, Directrice de recherche\, IBGC\, CNRS\, Université de Bordeaux\, Co-Directrice de Thèse\n\n
URL:https://www.bordeaux-neurocampus.fr/en/event/thesis-defense-christer-lohk/
CATEGORIES:Thesis
END:VEVENT
END:VCALENDAR