BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Bordeaux Neurocampus - ECPv4.9.10//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-WR-CALNAME:Bordeaux Neurocampus
X-ORIGINAL-URL:https://www.bordeaux-neurocampus.fr/en/
X-WR-CALDESC:Events for Bordeaux Neurocampus
BEGIN:VTIMEZONE
TZID:Europe/Paris
BEGIN:DAYLIGHT
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
TZNAME:CEST
DTSTART:20250330T010000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:+0200
TZOFFSETTO:+0100
TZNAME:CET
DTSTART:20251026T010000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;TZID=Europe/Paris:20251204T150000
DTEND;TZID=Europe/Paris:20251204T150000
DTSTAMP:20260430T232055
CREATED:20251118T095115Z
LAST-MODIFIED:20251124T185916Z
UID:189874-1764860400-1764860400@www.bordeaux-neurocampus.fr
SUMMARY:Thesis defense - Anna Saint-Jean
DESCRIPTION:Venue : centre Broca \n\nAnna SAINT-JEAN\nTeam : Cortical plasticity \nNeurocentre Magendie \nTitle\nElectrophysiologic\, transcriptomic and morphologic plasticity of spinal GABAergic interneurons to decipher atypical mechanosensory perception in autism. \nAbstract\nAutism is characterized by impairments in social communication and interactions\, as well as repetitive behaviours. Most people with autism also exhibit aberrant reactivity to sensory stimuli including tactile stimuli. In addition to being a major source of discomfort for autistic people\, recent research suggests that tactile dysfunction may actually contribute to the development of the others core symptoms. This is why understanding the neural mechanisms behind tactile dysfunction is so important. Tactile information is perceived in the periphery and processed in the spinal cord under the control of inhibitory interneurons before being sent to higher brain structures. However\, whether these inhibitory neurons undergo functional alterations in autistic models remain poorly understood. To explore the functional plasticity of spinal GABAergic interneurons (SGI) in autism\, we used Fmr1-/y mice crossed with Gad1-GFP reporter lines. These mice exhibit haploinsufficiency for the Gad1 gene\, which may interfere with the development of characteristic autistic phenotypes. We show that Fmr1-/y-Gad1-GFP mice display tactile deficits (longer latencies in tape removal test\, decreased reactivity to brush stimulation)\, decreased anxiety in elevated plus maze\, and social deficits\, as typically observed in autistic models. Then\, to characterize electrophysiologic\, transcriptomic and morphologic properties of SGI in acute spinal slices we used the patch-seq technique. We observed that SGI exhibit altered excitability\, characterized by an increased firing frequency of action potentials (AP) at low current injections\, but a reduced frequency at high intensities compared to WT mice. This results in a narrower functional dynamic range\, shifted toward lower-intensity stimulations. Importantly\, transcriptomic analysis of recorded neurons reveals that these changes are restricted to a subpopulation of SGI expressing the parvalbumin (Pvalb) and the neuropeptide Y (NPY) marker. Altogether\, we suggest that functional alterations of SGI\, particularly Pvalb and NPY neurons in autism models may disrupt tactile information processing upstream of the brain and may play a critical role in the development of sensory\nand social deficits characteristic of autism\, underscoring their potential as therapeutic targets. Keywords Autism\, spinal cord\, GABAergic interneurons\, patch-seq\, electrophysiology\, Fragile X Syndrome. \nJury\nMme Ingrid BUREAU\, Chargée de Recherche\, IBDM Marseille Rapportrice\nMr Eric LINGUEGLIA\, Directeur de Recherche\, IPMC Nice Rapporteur\nMr Alexandre FAVEREAUX\, Professeur de l’Université de Bordeaux\, IINS Bordeaux Examinateur\nMr Amaury FRANCOIS\, Chargé de Recherche\, IGF Montpellier Examinateur\nMme Graziella DI CRISTO\, Professeure de l’Université de Montréal\, CHU Sainte Justine Examinatrice\nMme Matilde CORDERO-ERAUSQUIN\, Directrice de Recherche\, INCI Strasbourg Examinatrice\nMr Yves LE FEUVRE\, Chargé de Recherche\, Neurocentre Magendie Bordeaux Directeur de thèse \n  \n  \n
URL:https://www.bordeaux-neurocampus.fr/en/event/thesis-defense-anna-saint-jean/
CATEGORIES:Thesis
END:VEVENT
END:VCALENDAR