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X-WR-CALDESC:Events for Bordeaux Neurocampus
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DTSTART;TZID=Europe/Paris:20240321T160000
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UID:170086-1711036800-1711036800@www.bordeaux-neurocampus.fr
SUMMARY:Seminar - Francesca Pennacchietti
DESCRIPTION:Venue: Centre Broca \n\n Francesca Pennacchietti\nPostdoctoral researcher in Ilaria Testa’s team\nKTH Royal Institute of Technology\, Stockholm\, Sweden \nInvited by Gregory Giannone and Jean-Baptiste Sibarita \nTitle\nQuantitative live-cell imaging at the nanoscale \nAbstract\nObserving the interplay of organelles and macromolecular complexes inside living cells and tissues calls for the continuous development of minimally invasive optical systems performing at high spatio-temporal resolution. Reversibly photoswitchable fluorescent proteins (rsFPs) play a key role as tags in optical nanoscopy. Their characteristic state transition between a long-lived dark state (OFF) and a fluorescent state (ON) allows to decrease the intensities required for imaging by several orders of magnitude compared to short-lived electronic transitions. Consequently\, techniques such as REversible Saturable/Switchable OpticaL Fluorescence Transition microscopy (RESOLFT)\, when combined with rsFP switching\, are ideal for non-invasive investigations of living systems at the nanoscale. \nIn this talk\, I will highlight how the switching properties of the rsFPs are the major driving forces in the development of new imaging schemes for faster and gentler nanoscopy and how they can extend the level of information accessible from a super-resolution microscope. \n
URL:https://www.bordeaux-neurocampus.fr/en/event/seminar-francesca-pennacchietti/
CATEGORIES:For scientists,home-event,Impromptu seminar
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