Tools for investigating peptide-protein interactions: Peptide incorporation of environment-sensitive fluorophores through spps-based ‘building block’ approach
Nat Protoc. 2007-12-01; 2(12): 3210-3218
DOI: 10.1038/nprot.2007.443
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1. Nat Protoc. 2007;2(12):3210-8.
Tools for investigating peptide-protein interactions: peptide incorporation of
environment-sensitive fluorophores through SPPS-based ‘building block’ approach.
Sainlos M(1), Imperiali B.
Author information:
(1)Department of Chemistry, Massachusetts Institute of Technology, 77
Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.
This protocol presents the synthesis and peptide incorporation of
environment-sensitive fluorescent amino acids derived from the
dimethylamino-phthalimide family. The procedure uses anhydride precursors of
4-dimethylaminophthalimide (4-DMAP) or 6-dimethylaminonaphthalimide (6-DMN),
whose syntheses are described in a related protocol by these authors. In this
study, the corresponding fluorescent amino acids can be readily obtained in
Fmoc-protected form for convenient use as building blocks in solid phase peptide
synthesis (SPPS). The time required to complete the procedure depends on the size
and the number of peptides targeted. Alternatively, the chromophores can be
incorporated directly after SPPS via on-resin derivatization of peptides, which
is an option described in a related protocol by these authors.
DOI: 10.1038/nprot.2007.443
PMID: 18079721 [Indexed for MEDLINE]