Segregation of dopamine and glutamate release sites in dopamine neuron axons: regulation by striatal target cells.
FASEB j.. 2018-07-16; 33(1): 400-417
DOI: 10.1096/fj.201800713rr
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Fortin GM(1), Ducrot C(1)(2), Giguère N(1)(2), Kouwenhoven WM(1), Bourque MJ(1), Pacelli C(1), Varaschin RK(1), Brill M(1), Singh S(3), Wiseman PW(3), Trudeau
LÉ(1)(2)(4).
Author information:
(1)Department of Pharmacology and Physiology, Université de Montréal, Montréal, Quebec, Canada.
(2)Department of Neurosciences, Université de Montréal, Montreal, Quebec, Canada.
(3)Department of Chemistry, McGill University, Montreal, Quebec, Canada; and.
(4)Groupe de Recherche sur le Système Nerveux Central, Faculty of Medicine, Université de Montréal, Montréal, Quebec, Canada.
Dopamine (DA) is a key regulator of circuits controlling movement and motivation.
A subset of midbrain DA neurons has been shown to express the vesicular glutamate
transporter (VGLUT)2, underlying their capacity for glutamate release. Glutamate
release is found mainly by DA neurons of the ventral tegmental area (VTA) and can
be detected at terminals contacting ventral, but not dorsal, striatal neurons,
suggesting the possibility that target-derived signals regulate the
neurotransmitter phenotype of DA neurons. Whether glutamate can be released from
the same terminals that release DA or from a special subset of axon terminals is
unclear. Here, we provide in vitro and in vivo data supporting the hypothesis
that DA and glutamate-releasing terminals in mice are mostly segregated and that
striatal neurons regulate the cophenotype of midbrain DA neurons and the
segregation of release sites. Our work unveils a fundamental feature of dual
neurotransmission and plasticity of the DA system.-Fortin, G. M., Ducrot, C.,
Giguère, N., Kouwenhoven, W. M., Bourque, M.-J., Pacelli, C., Varaschin, R. K.,
Brill, M., Singh, S., Wiseman, P. W., Trudeau, L.-E. Segregation of dopamine and
glutamate release sites in dopamine neuron axons: regulation by striatal target
cells.