Regulatory peptides in fruit fly midgut
Cell Tissue Res. 2008-10-30; 334(3): 499-516
DOI: 10.1007/s00441-008-0708-3
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1. Cell Tissue Res. 2008 Dec;334(3):499-516. doi: 10.1007/s00441-008-0708-3. Epub
2008 Oct 30.
Regulatory peptides in fruit fly midgut.
Veenstra JA(1), Agricola HJ, Sellami A.
Author information:
(1)CNIC UMR 5228 CNRS, Université Bordeaux 1, 33400, Talence, France.
Regulatory peptides were immunolocalized in the midgut of the fruit fly
Drosophila melanogaster. Endocrine cells were found to produce six different
peptides: allatostatins A, B and C, neuropeptide F, diuretic hormone 31, and the
tachykinins. Small neuropeptide-F (sNPF) was found in neurons in the hypocerebral
ganglion innervating the anterior midgut, whereas pigment-dispersing factor was
found in nerves on the most posterior part of the posterior midgut.
Neuropeptide-F (NPF)-producing endocrine cells were located in the anterior and
middle midgut and in the very first part of the posterior midgut. All NPF
endocrine cells also produced tachykinins. Endocrine cells containing diuretic
hormone 31 were found in the caudal half of the posterior midgut; these cells
also produced tachykinins. Other endocrine cells produced exclusively tachykinins
in the anterior and posterior extemities of the midgut.
Allatostatin-immunoreactive endocrine cells were present throughout the midgut.
Those in the caudal half of the posterior midgut produced allatostatins A,
whereas those in the anterior, middle, and first half of the posterior midgut
produced allatostatin C. In the middle of the posterior midgut, some endocrine
cells produced both allatostatins A and C. Allatostatin-C-immunoreactive
endocrine cells were particularly prominent in the first half of the posterior
midgut. Allatostatin B/MIP-immunoreactive cells were not consistently found and,
when present, were only weakly immunoreactive, forming a subgroup of the
allatostatin-C-immunoreactive cells in the posterior midgut. Previous work on
Drosophila and other insect species suggested that (FM)RFamide-immunoreactive
endocrine cells in the insect midgut could produce NPF, sNPF, myosuppressin,
and/or sulfakinins. Using a combination of specific antisera to these peptides
and transgenic fly models, we showed that the endocrine cells in the adult
Drosophila midgut produced exclusively NPF. Although the Drosophila insulin gene
Ilp3 was abundantly expressed in the midgut, Ilp3 was not expressed in endocrine
cells, but in midgut muscle.
DOI: 10.1007/s00441-008-0708-3
PMID: 18972134 [Indexed for MEDLINE]