Polyunsaturated fatty acids induce modification in the lipid composition and the prostaglandin production of the conjunctival epithelium cells
Graefes Arch Clin Exp Ophthalmol. 2011-09-06; 250(2): 211-222
DOI: 10.1007/s00417-011-1801-y
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1. Graefes Arch Clin Exp Ophthalmol. 2012 Feb;250(2):211-22. doi:
10.1007/s00417-011-1801-y. Epub 2011 Sep 6.
Polyunsaturated fatty acids induce modification in the lipid composition and the
prostaglandin production of the conjunctival epithelium cells.
Viau S(1), Leclère L, Buteau B, Grégoire S, Acar N, Bron A, Creuzot-Garcher CP,
Bretillon L, Joffre C.
Author information:
(1)UMR CSGA 1324 INRA–6265 CNRS–Université de Bourgogne–AgroSup Dijon, Eye and
Nutrition Research Group, 21000 Dijon, France.
BACKGROUND: This study was conducted to evaluate whether polyunsaturated fatty
acids (PUFA) such as γ-linolenic acid (GLA) and eicosapentaenoic acid (EPA), as
found in the diet, may affect the lipid composition of conjunctival epithelium
and whether these modifications affect prostaglandin (PG) production after
inflammatory stimulation.
METHODS: Chang and IOBA-NHC conjunctival human cells were treated with GLA and/or
EPA at 5, 10, 20, 30, 40, or 50 μg/ml for 72 h and then were stimulated with
interferon-gamma (IFN-γ) for 48 h. Changes in the composition of neutral lipids
and phospholipids were monitored by gas chromatography. PGE1 and PGE2 levels were
measured by enzyme immunoassay.
RESULTS: PUFA supplementations in the culture medium induced incorporation of
these fatty acids and of their metabolites in neutral lipids and phospholipids of
the conjunctival cells. The fatty acid composition of neutral lipids and
phospholipids was not affected by stimulation with IFN-γ. The production of PGE1
and PGE2 was affected by GLA supplementation whereas it was not modified by EPA
supplementation. A combined supplementation of EPA and GLA did not change the
production of PGE1 but decreased the production of PGE2.
CONCLUSIONS: These results suggest that modulation of fatty acid composition and
PG production by PUFA supplementation is possible in the conjunctival epithelium,
which is an important site of inflammation in dry eye syndrome.
DOI: 10.1007/s00417-011-1801-y
PMID: 21894532 [Indexed for MEDLINE]