Organellar protein complexes of Caco-2 human cells analyzed by two-dimensional blue native/SDS-PAGE and mass spectrometry
J. Proteome Res.. 2010-09-01; 9(10): 5093-5107
DOI: 10.1021/pr100381m
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1. J Proteome Res. 2010 Oct 1;9(10):5093-107. doi: 10.1021/pr100381m.
Organellar protein complexes of Caco-2 human cells analyzed by two-dimensional
blue native/SDS-PAGE and mass spectrometry.
Lasserre JP(1), Sylvius L, Joubert-Caron R, Caron M, Hardouin J.
Author information:
(1)Laboratoire de Biochimie des Protéines et Protéomique, Université Paris 13,
UMR CNRS 7033, 74 rue Marcel Cachin, Bobigny cedex, France.
The complexome is essential for a better understanding of protein functions. In
order to study protein complexes, an approach allowing the extraction and the
analysis in native conditions is needed. Two-dimensional blue native/SDS-PAGE (2D
BN/SDS-PAGE) technology is thus an interesting and powerful approach for this
purpose. This report deals with the analysis and the identification of the
organellar protein complexes of Caco-2 human cells using 2D BN/SDS-PAGE and
HPLC-chip-MS. We identified 58 protein complexes (26 heteromultimeric and 32
homomultimeric complexes) and 4 monomeric proteins. Among them, 32 protein
complexes were pointed out, providing insights into the function of previously
uncharacterized human proteins.
DOI: 10.1021/pr100381m
PMID: 20809636 [Indexed for MEDLINE]