Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models.
Biosensors and Bioelectronics. 2019-01-01; 124-125: 129-135
DOI: 10.1016/j.bios.2018.10.021
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Caluori G(1), Pribyl J(2), Pesl M(3), Jelinkova S(4), Rotrekl V(4), Skladal P(5), Raiteri R(6).
Author information:
(1)Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic; International Clinical Research Centre of Saint Anne Hospital Brno, Pekarska 53, 60200 Brno, Czech Republic; Department of Informatics, Bioengineering Robotics and Systems Engineering, University of
Genova, Via All’Opera Pia, 13, 16145 Genova, Italy.
(2)Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
(3)International Clinical Research Centre of Saint Anne Hospital Brno, Pekarska 53, 60200 Brno, Czech Republic; Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic; 1st Department of Cardiovascular Diseases, St. Anne’s University Hospital and Masaryk University, Pekarska 53, Brno, Czech Republic.
(4)Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
(5)Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic. Electronic address: .
(6)Department of Informatics, Bioengineering Robotics and Systems Engineering, University of Genova, Via All’Opera Pia, 13, 16145 Genova, Italy. Electronic address: .
Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously
probed with cantilever from atomic force microscope (AFM) system. This electric /
nanomechanical combination in real time recorded beating force of the CMs cluster
and the triggering electric events. Such “organ-on-a-chip” represents a tool for
drug development and disease modeling. The human pluripotent stem cells included
the WT embryonic line CCTL14 and the induced dystrophin deficient line
reprogrammed from fibroblasts of a patient affected by Duchenne Muscular
Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated
to CMs and employed with the AFM/MEA platform for diseased CMs’ drug response
testing and DMD characterization. The dependence of cardiac parameters on
extracellular Ca2+ was studied. The differential evaluation explained the
observed effects despite variability of biological samples. The β-adrenergic
stimulation (isoproterenol) and antagonist trials (verapamil) addressed
ionotropic and chronotropic cell line-dependent features. For the first time, a
distinctive beating-force relation for DMD CMs was measured on the 3D cardiac in
vitro model.
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