High-content super-resolution imaging of live cell by uPAINT.

Grégory Giannone, Eric Hosy, Jean-Baptiste Sibarita, Daniel Choquet, Laurent Cognet
Nanoimaging. 2012-09-18; : 95-110
DOI: 10.1007/978-1-62703-137-0_7

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1. Methods Mol Biol. 2013;950:95-110. doi: 10.1007/978-1-62703-137-0_7.

High-content super-resolution imaging of live cell by uPAINT.

Giannone G(1), Hosy E, Sibarita JB, Choquet D, Cognet L.

Author information:
(1)Interdisciplinary Institute for Neuroscience, Université de Bordeaux,
Bordeaux, France.

In this chapter, we present the uPAINT method (Universal Point Accumulation
Imaging in Nanoscale Topography), a simple single-molecule super-resolution
method which can be implemented on any wide field fluorescence microscope
operating in oblique illumination. The key feature of uPAINT lies in recording
high numbers of single molecules at the surface of a cell by constantly labeling
while imaging. In addition to generating super-resolved images, uPAINT can
provide dynamical information on a single live cell with large statistics
revealing localization-specific diffusion properties of membrane biomolecules.
Interestingly, any membrane biomolecule that can be labeled with a fluorescent
ligand can be studied, making uPAINT an extremely versatile method.

DOI: 10.1007/978-1-62703-137-0_7
PMID: 23086872 [Indexed for MEDLINE]

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