Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach

Huanchao Zeng, Yiru Qin, Erxia Du, Qiulan Wei, Ying Li, Danyan Huang, Guirong Wang, Jan A. Veenstra, Sheng Li, Na Li
J. Proteome Res.. 2020-11-09; 20(2): 1217-1228
DOI: 10.1021/acs.jproteome.0c00596

PubMed
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Zeng H(1)(2), Qin Y(1)(2), Du E(1)(2), Wei Q(1), Li Y(1), Huang D(1), Wang G(3), Veenstra JA(4), Li S(1)(2), Li N(1)(2).

Author information:
(1)Guangdong Provincial Key Laboratory of Insect Developmental Biology and
Applied Technology, Institute of Insect Science and Technology, School of Life
Sciences, South China Normal University, Guangzhou 510631, China.
(2)Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect
Developmental Biology and Applied Technology, South China Normal University,
Meizhou 514779, China.
(3)Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis
Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at
Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China.
(4)INCIA, UMR 5287 CNRS, Université de Bordeaux, Pessac F33615, France.

As a model hemimetabolous insect species and an invasive urban pest that is
globally distributed, the American cockroach, Periplaneta americana, is of great
interest in both basic and applied research. Previous studies on P. americana
neuropeptide identification have been based on biochemical isolation and
molecular cloning. In the present study, an integrated approach of genomics- and
peptidomics-based discovery was performed for neuropeptide identification in this
insect species. First, 67 conserved neuropeptide or neurohormone precursor genes
were predicted via an in silico analysis of the P. americana genome and
transcriptome. Using a large-scale peptidomic analysis of peptide extracts from
four different tissues (the central nervous system, corpora cardiac and corpora
allata complex, midgut, and male accessory gland), 35 conserved (predicted)
neuropeptides and a potential (novel) neuropeptide were then identified.
Subsequent experiments revealed the tissue distribution, sex difference, and
developmental patterns of two conserved neuropeptides (allatostatin B and short
neuropeptide F) and a novel neuropeptide (PaOGS36577). Our study shows a
comprehensive neuropeptidome and detailed spatiotemporal distribution patterns,
providing a solid basis for future functional studies of neuropeptides in the
American cockroach (data are available via ProteomeXchange with identifier
PXD021660).

 

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