Characterization of the mouse adeno-associated virus AAVS1 ortholog.
Journal of Virology. 2004-07-27; 78(16): 8917-8921
DOI: 10.1128/jvi.78.16.8917-8921.2004
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1. J Virol. 2004 Aug;78(16):8917-21.
Characterization of the mouse adeno-associated virus AAVS1 ortholog.
Dutheil N(1), Yoon-Robarts M, Ward P, Henckaerts E, Skrabanek L, Berns KI,
Campagne F, Linden RM.
Author information:
(1)Carl C. Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai
School of Medicine, One Gustave L. Levy Pl., Box 1496, New York, NY 10029, USA.
The nonpathogenic human adeno-associated virus (AAV) has developed a mechanism to
integrate its genome into human chromosome 19 at 19q13.4 (termed AAVS1), thereby
establishing latency. Here, we provide evidence that the chromosomal signals
required for site-specific integration are conserved in the mouse genome proximal
to the recently identified Mbs85 gene. These sequence motifs can be specifically
nicked by the viral Rep protein required for the initiation of site-specific AAV
DNA integration. Furthermore, these signals can serve as a minimal origin for
Rep-dependent DNA replication. In addition, we isolated the mouse Mbs85 proximal
promoter and show transcriptional activity in three mouse cell lines.
DOI: 10.1128/JVI.78.16.8917-8921.2004
PMCID: PMC479059
PMID: 15280500 [Indexed for MEDLINE]