Architecture and permeability of post-cytokinesis plasmodesmata lacking cytoplasmic sleeves
NPLANTS. 2017-06-12; 3(7): 17082
DOI: 10.1038/nplants.2017.82
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1. Nat Plants. 2017 Jun 12;3:17082. doi: 10.1038/nplants.2017.82.
Architecture and permeability of post-cytokinesis plasmodesmata lacking
cytoplasmic sleeves.
Nicolas WJ(1), Grison MS(1), Trépout S(2), Gaston A(1), Fouché M(1), Cordelières
FP(3), Oparka K(4), Tilsner J(5)(6), Brocard L(7), Bayer EM(1).
Author information:
(1)Laboratory of Membrane Biogenesis, UMR5200 CNRS, University of Bordeaux, 71
Avenue Edouard Bourlaux, 33883 Villenave d’Ornon Cedex, France.
(2)Institut Curie, Centre de Recherche, Bât. 112, Centre Universitaire, 91405
Orsay Cedex, France.
(3)Bordeaux Imaging Centre, UMS 3420 CNRS, CNRS-INSERM-University of Bordeaux
146, rue Léo Saignat, 33076 Bordeaux Cedex, France.
(4)Institute of Molecular Plant Sciences, University of Edinburgh, Edinburgh EH9
3BF, UK.
(5)Biomedical Sciences Research Complex, University of St Andrews, St Andrews
KY16 9ST, UK.
(6)Cell and Molecular Sciences, James Hutton Institute, Dundee DD2 5DA, UK.
(7)Bordeaux Imaging Centre, Plant Imaging Plateform, UMS 3420,
INRA-CNRS-INSERM-University of Bordeaux, 71 Avenue Edouard Bourlaux, 33883
Villenave-d’Ornon Cedex, France.
Plasmodesmata are remarkable cellular machines responsible for the controlled
exchange of proteins, small RNAs and signalling molecules between cells. They are
lined by the plasma membrane (PM), contain a strand of tubular endoplasmic
reticulum (ER), and the space between these two membranes is thought to control
plasmodesmata permeability. Here, we have reconstructed plasmodesmata
three-dimensional (3D) ultrastructure with an unprecedented level of 3D
information using electron tomography. We show that within plasmodesmata, ER-PM
contact sites undergo substantial remodelling events during cell differentiation.
Instead of being open pores, post-cytokinesis plasmodesmata present such intimate
ER-PM contact along the entire length of the pores that no intermembrane gap is
visible. Later on, during cell expansion, the plasmodesmata pore widens and the
two membranes separate, leaving a cytosolic sleeve spanned by tethers whose
presence correlates with the appearance of the intermembrane gap. Surprisingly,
the post-cytokinesis plasmodesmata allow diffusion of macromolecules despite the
apparent lack of an open cytoplasmic sleeve, forcing the reassessment of the
mechanisms that control plant cell-cell communication.
DOI: 10.1038/nplants.2017.82
PMID: 28604682 [Indexed for MEDLINE]