Turning up the Green Light

Trends Cell Biol. 2021 Mar;31(3):143-145. doi: 10.1016/j.tcb.2020.12.007. Epub 2020 Dec 29.

Abstract

Fluorescent proteins (FPs) have become indispensable tools in biological research for labeling cells and proteins and sensing their biochemical activity. By introducing 'folding mutations', Campbell et al. engineered a new GFP variant with dramatically enhanced cellular brightness and stability, facilitating advanced cellular bioimaging applications in neuroscience and beyond.

Keywords: brain imaging; fluorescent proteins; protein engineering; super-resolution microscopy.

MeSH terms

  • Green Fluorescent Proteins* / genetics
  • Mutation

Substances

  • Green Fluorescent Proteins