Cyclic AMP-modulated potassium channels in murine B cells and their precursors

Science. 1987 Mar 6;235(4793):1211-4. doi: 10.1126/science.2434998.

Abstract

A voltage-dependent potassium current (the delayed rectifier) has been found in murine B cells and their precursors with the whole-cell patch-clamp technique. The type of channel involved in the generation of this current appears to be present throughout all stages of pre-B-cell differentiation, since it is detected in pre-B cell lines infected with Abelson murine leukemia virus; these cell lines represent various phases of B-cell development. Thus, the presence of this channel is not obviously correlated with B-cell differentiation. Although blocked by Co2+, the channel, or channels, does not appear to be activated by Ca2+ entry. It is, however, inactivated by high intracellular Ca2+ concentrations. In addition, elevation of intracellular adenosine 3', 5'-monophosphate induces at all potentials a rapid decrease in the peak potassium conductance and increased rates of activation and inactivation. Therefore, potassium channels can be physiologically modulated by second messengers in lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / metabolism*
  • B-Lymphocytes / physiology
  • Calcium / pharmacology
  • Cell Differentiation
  • Colforsin / pharmacology
  • Cyclic AMP / physiology*
  • Guanosine Triphosphate / pharmacology
  • Immunocompetence
  • Ion Channels / drug effects*
  • Ion Channels / metabolism
  • Lipopolysaccharides / pharmacology
  • Mice
  • Potassium / metabolism*

Substances

  • Ion Channels
  • Lipopolysaccharides
  • Colforsin
  • Guanosine Triphosphate
  • Cyclic AMP
  • Potassium
  • Calcium