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L’imagerie super résolution s’applique aussi au végétal !

Le 3 février 2015

Molecular Plant  / Letter to the Editor
Super-Resolved and Dynamic Imaging of Membrane Proteins in Plant Cells Reveal Contrasting Kinetic Profiles and Multiple Confinement Mechanisms
Mol Plant. 2014 Oct 24. pii: ssu123. [Epub ahead of print]

   Eric Hosy 2, 3, Alexandre Martinière 1, Daniel Choquet 2, 3, Christophe Maurel 1, Doan-Trung Luu 1,

1) Biochimie et Physiologie Moléculaire des Plantes, Institut de Biologie Intégrative des Plantes, UMR 5004 CNRS/UMR 0386 INRA/Montpellier SupAgro/Université Montpellier 2, F-34060 Montpellier Cedex 2, France
2) Interdisciplinary Institute for Neuroscience, University of Bordeaux, F-33077 Bordeaux Cedex, France
3) Centre National de la Recherche Scientifique Unité Mixte de Recherche 5297, F-33000 Bordeaux, France

Dear Editor,

Microscopic techniques allow either a global mobility analysis of proteins with fluorescence recovery after photobleaching (FRAP) or single-protein mobility characterization with single-particle or quantum dot tracking. For instance, total internal reflection fluorescence microscopy allowed single-particle tracking (SPT) of Arabidopsis plasma membrane (PM) proteins, revealing their heterogeneous distribution, low lateral diffusion, and dynamic properties in response to salt stress ( Li et al., 2011)Studies of SPT based on green fluorescent protein are unfortunately restricted by the density of proteins at the surface, since diffracted emission fluorescence prevents tracking of individual proteins separated by less than 1 μm. The recent emergence of high-density SPT techniques based on temporal emission decorrelation, such as single-particle tracking with photoactivated localization microscopy (sptPALM), allowed the diffraction limit of classic light microscopy to be broken and reach nanometer-level spatial resolutions .......  lire la suite 

......In conclusion, the present study reports, for the first time, the application of the sptPALM technique to plant cells to decipher the dynamic organization of both tonoplast and PM proteins in live cells. It also suggests that super-resolution techniques could be used to address other fundamental processes in plant cells.