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Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.

Martineau M, Somasundaram A, Grimm JB, Gruber TD, Choquet D, Taraska JW, Lavis LD, Perrais D.
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.
Nat Commun. 2017 Nov 10;8(1):1412.

Abstract

The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.

 

doi: 10.1038/s41467-017-01752-5

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