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Séminaire - Andy GreenhalghThe interplay of microglia and monocyte derived macrophages after traumatic spinal cord injury

Abstract :

Dr Andy Greenhalgh (Postdoctoral Research Fellow, Centre for Research in Neuroscience, The Research Institute of the McGill University Health Centre) 

 Traumatic spinal cord injury (SCI) leads to the life-changing consequences of paralysis, sensory loss, and other functional impairments below the level of injury.
The physical trauma and haemorrhage cause direct damage to spinal cord tissue and triggers a cascade of cellular responses, including inflammation, which are initiated within minutes and last for several months. The main focus of our research is twofold: 1) to understand the triggers of harmful, sustained inflammation and 2) investigate the actions and interactions of the two main immune cells mediating this inflammation: CNS resident microglia and monocyte derived macrophages. It is now recognised that microglia and monocyte derived macrophages are ontogenetically distinct, and we have shown that they perform temporally different functions after SCI. Our latest work aims to investigate the potential cross-talk between microglia and infiltrating cells. We have, therefore, developed and characterised in vitro cultures of adult mouse microglia in order to assess macrophage impact on their actions. This system may help us to identify novel pathways of inflammatory signalling which can be targeted to improve the timely resolution of inflammation after SCI.

Selected publications

Greenhalgh AD, David S (2014). Differences in the phagocytic response of microglia and peripheral macrophages after spinal cord injury and its effects on cell death. J Neurosci. 2014 Apr 30;34(18):6316-22

Kroner A, Greenhalgh AD, Zarruk JG, Passos Dos Santos R, Gaestel M, David S (2014). TNF and increased intracellular iron alter macrophage polarization to a detrimental M1 phenotype in the injured spinal cord. Neuron. 2014 Sep 3;83(5):1098-116.

Greenhalgh AD, Brough D, Robinson EM, Girard S, Rothwell NJ, Allan SM (2012) Interleukin-1 receptor antagonist is beneficial in rat subarachnoid haemorrhage by blocking haem driven inflammation. Disease Models & Mechanisms 6:823-33