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Séminaire impromptu - Jun NishiyamaDissecting Molecular Networks in Synapse: From Genome Editing to FRET Imaging

Abstract :


Our brain functions depend on proper connections between tens of billions of neurons.
These connections or synapses are considered to be disrupted in many neuropsychiatric disorders, such as autism, schizophrenia, and mental retardation. However, detailed molecular mechanisms underlying synaptic function and dysfunction are largely unknown. In this seminar, I will introduce my recent studies to probe endogenous proteins and image signal transduction in single synapses using CRISPR/Cas9-mediated genome editing and various optical techniques. In addition, I will discuss how these advanced tools can help for the deeper understanding of molecular regulation of synapse structure and function.

Selected publications

1) Nishiyama, J.*, Mikuni, T*,†., Yasuda R†. Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain. Neuron, 96, 755-768 (2017).

2) Mikuni, T.*, Nishiyama, J.*,†, Sun Y., Kamasawa N., Yasuda, R.† High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing. Cell, 165 1803-17 (2016).

3) Nishiyama, J. and Yasuda, R. Biochemical Computation for Spine Structural Plasticity. Neuron, 87 63-75 (2015).