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Séminaire - Carsten WotjakIn vivo imaging of memory traces in mice

Abstract :

Remarkable technical developments enable the visualization of learning-related changes in neuronal activity and synaptic morphology in defined brain structures in living animals. In contrast, more holistic approaches aimed at identifying the complex matrix of different brain structures implicated in a given learning task are still in their infancies. Here I will introduce manganese-enhanced magnetic resonance imaging (MEMRI) in combination with whole brain voxel-wise analysis as an interesting tool for visualizing memory traces in vivo. I will provide evidence for activity-dependent uptake and axonal transport of Mn2+, and demonstrate the suitability of the approach for detecting brain structures specifically implicated in different strategies of spatial navigation.

Selected publications

Kleinknecht KR, Bedenk BT, Kaltwasser SF, Gruenecker B, Yen YC, Czisch M, Wotjak CT (2012) Hippocampus-dependent place learning enables spatial flexibility in mice. Front Behav Neuroscience 6:87 (doi:10.3389/fnbeh.2012.00087)

Reichel JM, Bedenk BT, Czisch M, Wotjak CT (2017) Age-related cognitive decline coincides with accelerated volume loss of the dorsal but not ventral hippocampus in mice. Hippocampus Jan;27(1):28-35.

Bedenk BT, Almeida-Corrêa S, Jurik A, Dedic N, Grünecker B, Genewsky AJ, Kaltwasser SF, Riebe CJ, Deussing JM, Czisch M, Wotjak CT (2018) Mn(2+) dynamics in manganese-enhanced MRI (MEMRI): Ca(v)1.2 channel-mediated uptake and preferential accumulation in projection terminals. Neuroimage 169: 374-382.

Almeida-Correa S, Czisch M, Wotjak CT (2018). In Vivo Visualization of Active Polysynaptic Circuits With Longitudinal Manganese-Enhanced MRI (MEMRI). Front Neural Circuits 12, 42.