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Événement - Mini-symposium "Morphogenesis, adhesion and cytoskeleton in neurons"

Abstract :

Contacts : Mireille Montcouquiol & Nathalie Sans, INSERM U1215, Neurocentre Magendie,
Supported by the Neurocentre Magendie, INSERM U1215, the LABEX BRAIN and the Bordeaux Neurocampus / 1er dec. 2016 à 09:00 (Institut Francois Magendie)

This will be a good opportunity for you to learn a bit more about neuronal development and themes such as axonal guidance, neuronal outgrowth and engineered microenvironments for cell based assays.

Fanny Mann (Institut de Biologie du Développement de Marseille, France) The double game of Semaphorins in neuronal wiring

The group of Fanny Mann focuses on the Semaphorin (Sema) family of molecules in the control of axonal guidance and have highlighted the influence of two members of this protein family, Sema3C and Sema3E, in the establishment of major brain fiber tracts.

Chauvet S, Mire E, Mann F (2017) Characterizing Semaphorin Signaling Using Isolated Neurons in Culture. Methods Mol Biol. 1493:223-235.

Deloulme JC, Gory-Fauré S, Mauconduit F, Chauvet S, Jonckheere J, Boulan B, Mire E, Xue J, Jany M, Maucler C, Deparis AA, Montigon O, Daoust A, Barbier EL, Bosc C, Deglon N, Brocard J, Denarier E, Le Brun I, Pernet-Gallay K, Vilgrain I, Robinson PJ, Lahrech H, Mann F, Andrieux A. Microtubule-associated protein 6 mediates neuronal connectivity through Semaphorin 3E-dependent signalling for axonal growth (2015) Nat Commun. 6:7246

Esther Stoeckli (Institute of Molecular Life Sciences,
Univ. Zurich, Switzerland) How to navigate an intermediate target during neural circuit formation

Stoeckli work focuses on the molecular mechanisms that underlie the establishment of neuronal circuits. In particular, she is interested in the molecular guidance cues that help axons, the long processes of neurons, to navigate through the preexisting tissue to their target cells.

Wilson NH & Stoeckli ET (2013) Sonic hedgehog regulates its own receptor on postcrossing commissural axons in a glypican1-dependent manner. Neuron, 79(3): 478-491

Frei JA & Stoeckli ET (2016) SynCAMs - From axon guidance to neurodevelopmental disorders. Mol Cell Neurosci. 2016 Sep 1. pii: S1044-7431(16)30139-7.

Alther TA, Domanitskaya E, Stoeckli ET (2016) Calsyntenin 1-mediated trafficking of axon guidance receptors regulates the switch in axonal responsiveness at a choice point. Development (2016), 143(6): 994-1004

Vincent Studer (IINS, UMR CNRS 5297, Bordeaux, France)

Engineered microenvironments for cell based assays The main objectives of the research of Vincent Studer are to provide the research community with imaging techniques to quantify cellular dynamics at high spatial and temporal resolutions, notably microfluidic devices or photopattern. These devices can probe the directional sensing of nerve growth cones in controlled chemical landscapes, test the combinatorial effect of multiple guidance cues on axon guidance, or evaluate the adhesion a synthetic substrate.

Strale PO, Azioune A, Bugnicourt G, Lecomte Y, Chahid M, Studer V (2016) Multiprotein Printing by Light-Induced Molecular Adsorption. Adv Mater. 28(10):2024-9

Dupin I, Lokmane L, Dahan M, Garel S, Studer V (2015) Subrepellent doses of Slit1 promote Netrin-1 chemotactic responses in subsets of axons. Neural Dev. 20;10:5

 11h-11h30 Coffee pause

Froylan Calderon de Anda 
(University Medical Center Hamburg-Eppendorf (UKE), Germany) Radial organization of F-actin in developing neurons

The group of F. Calderon de Anda is interest in how neurons acquire their morphology, notably axon and dendrites specification and their relationship to neuropsychiatric diseases like autism spectrum disorders.

Kwan V, Meka DP, White SH, Hung CL, Holzapfel NT, Walker S, Murtaza N, Unda BK, Schwanke B, Yuen RK, Habing K, Milsom C, Hope KJ, Truant R, Scherer SW, Calderon de Anda F, Singh KK (2016)

DIXDC1 Phosphorylation and Control of Dendritic Morphology Are Impaired by Rare Genetic Variants. Cell Rep. 2016 Nov 8;17(7):1892-1904. Calderon de Anda F, Madabhushi R, Rei D, Meng J, Gräff J, Durak O, Meletis K, Richter M, Schwanke B, Mungenast A, Tsai L-H (2016)

Cortical neurons gradually attain a post-mitotic state. Cell Research:1-15. Calderon de Anda F, Rosario AL, Durak O, Graff J, Meletis K, Rei D, Soda T, Madabhushi R, Ginty DD, Kolodkin A L, Tsai LH (2012) Autism spectrum disorder susceptibility gene TAOK2 affects basal dendrite formation in the neocortex. Nature Neurosci, 15,1022-1031.

Olivier Thoumine (IINS, UMR CNRS 5297, Bordeaux, France)

Mechanical clutch between the actin flow and N-cadherin adhesions in neuronal growth cones The group of O. Thoumine is interested in biomimetic systems coupled with high resolution imaging and predictive biophysical models, to probe the role of the cytoskeleton and adhesion proteins in growth cone motility and synaptogenesis.

Chamma I, Letellier M, Butler C, Tessier B, Lim KH, Gauthereau I, Choquet D, Sibarita JB, Park S, Sainlos M, Thoumine O (2016) Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin. Nat Commun. 7:10773.

Garcia M, Leduc C, Lagardère M, Argento A, Sibarita JB, Thoumine O (2015) Two-tiered coupling between flowing actin and immobilized N-cadherin/catenin complexes in neuronal growth cones. Proc Natl Acad Sci U S A. 112(22):6997-7002.

12h50-13h20 Nathalie Sans
(Neurocentre Magendie, INSERM1215, Bordeaux, France)
of the Planar Cell Polarity signaling pathway in the dynamic organization of dendritic spines

The group of Nathalie Sans focusses on the molecular mechanism controlled by planar cell polarity (PCP) signalling, a developmental pathway important for cell and tissue morphogenesis. She addresses the role of PCP genes in developmental and mature neurons, focusing on cytoskeletal organization, the main output of PCP signalling.

Hilal M, Moreau MM, Racca C, Pinheiro VL, et al., & Montcouquiol M*, Oliet SHR* & Sans N*. Activity-dependent neuroplasticity induced by an enriched environment reverses cognitive deficits in Scribble1 deficient mouse. Cerebral Cortex, in press.

Piguel NH, Fievre S, Blanc JM, Carta M, Moreau MM, Moutin E, Pinheiro VL, Medina C, Ezan J, Lasvaux L, Loll F, Durand CM, Chang K, Petralia RS, Wenthold RJ, Stephenson FA, Vuillard L, Darbon H, Perroy J, Mulle C, Montcouquiol M, Racca C, Sans N (2014)  Scribble1/AP2 complex coordinates NMDA receptor endocytic recycling. Cell Rep.9(2):712-27.

Ezan J, Lasvaux L, Gezer A, Novakovic A, May-Simera H, Belotti E, Lhoumeau AC, Birnbaumer L, Beer-Hammer S, Borg JP, Le Bivic A, Nürnberg B, Sans N, Montcouquiol M (2013) Primary cilium migration depends on G-protein signalling control of subapical cytoskeleton. Nat Cell Biol. 15(9):1107-15.