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Séminaire - Scott SternsonChemogenetic ion channel tools for neurobiology

Abstract :

Neuron activity is dependent on the concerted action of ion channels. Several years ago, we combined synthetic chemistry with protein engineering of ligand gated ion channel to develop pharmacologically selective ion channels with diverse conductance properties. These tools can be used to powerfully activate or inhibit neurons. I will discuss these engineered ligand gated ion channels and recent advances that we have made for their use in vivo.

Selected publications

1. D. Atasoy, Y. Aponte, H.H. Su, S.M. Sternson. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping. J. Neuroscience 28 (2008) 7025-30. 

 2. Y. Aponte, D. Atasoy, S.M. Sternson. AGRP neurons are sufficient to orchestrate feeding behavior rapidly and without training. Nature Neurosci. 13 (2011) 351-5. 

 3. D. Atasoy, J.N. Betley, H.H. Su, S.M. Sternson. Deconstruction of a neural circuit for hunger. Nature 488(2012) 172-7. 

 4. J.N. Betley, Z.F. Huang Cao, K.D. Ritola, S.M. Sternson. Parallel, redundant circuit organization for homeostatic control of feeding behavior. Cell 155 (2013) 137-50. 1

5.T.J. Stachniak, A. Ghosh, S.M. Sternson. Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus→midbrain pathway for feeding behavior. Neuron 82(2014) 797-808.

6. D. Atasoy, J.N. Betley, W-P. Li, H.H. Su, S.M. Sirtel, L.K. Scheffer, J.H. Simpson, R.D. Fetter, S.M. Sternson. A genetically specified connectomics approach applied to long-range feeding regulatory circuits. Nature Neurosci. 17, (2014) 1830-9.

7. J.N.cBetley, S. Xu, Z.F. Huang Cao, R. Gong, C. Magnus, Y. Yang, S.M. Sternson. Neurons for hunger and thirst transmit a negative valence teaching signal. Nature 521 (2015) 180-185.