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Séminaire - Bruno WeberIn vivo optical imaging of brain energy metabolism

Abstract :

Bruno Weber de l’Université de Zurich 

The lack of adequate methods to investigate brain energy metabolism with the required spatio-temporal resolution in the intact organism has hampered significant advances in the field. Förster resonance energy transfer (FRET) sensors specific for energy substrates, such as glucose, lactate and pyruvate have been developed and successfully used in cultured cells and in brain slices. A major advantage of these FRET sensors is that they do not interfere with the intrinsic metabolite concentrations and pathways. In addition to unsurpassed spatial resolution, FRET microscopy can also detect fast metabolic dynamics. Furthermore, these sensors have great potential for in vivo studies in combination with two-photon microscopy.

Selected publications

Barros LF, Sierralta J, Weber B (2015). How doth the little busy bee: unexpected metabolism. Trends in Neurosci, in press.

Luecker A, Weber B and Jenny P (2015). A dynamic model of oxygen transport from capillaries to tissue with moving red blood cells. AJP Heart and Circ Physiol, in press.

Ghosh A, Wyss MT, Weber B. Somatotopic astrocytic activity in the somatosensory cortex. Glia. 2013 Apr;61(4):601-10. doi: 10.1002/glia.22458. Epub 2013 Jan 22.

Hirsch S, Reichold J, Schneider M, Székely G, Weber B. Topology and hemodynamics of the cortical cerebrovascular system. J Cereb Blood Flow Metab. 2012 Jun;32(6):952-67. doi: 10.1038/jcbfm.2012.39. Epub 2012 Apr 4. Review.

Wyss MT, Jolivet R, Buck A, Magistretti PJ and Weber B. In vivo evidence for lactate as a neuronal energy source. J Neurosci. 2011 May 18;31(20):7477-85. doi: 10.1523/JNEUROSCI.0415-11.2011.

Scientific focus :

Our group uses a wide range of imaging tools to study the cell-to-cell communication pathways involved in energy metabolism and information processing in cerebral cortex. Furthermore, we are working on dissecting the interaction of neurons and astrocytes with the vascular system, which is responsible for maintaining adequate delivery of oxygen and energy substrates to the brain. As well as studying these systems, the development of imaging systems for in vivo research is an additional research focus of the group.