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Journal club - Journal Club SynapseProchain Journal Club : Mardi 4 mars 2014

Abstract :

La prochaine séance du JC sera le Mardi 04 mars 2014 à 13h30 (PGF sud)

Ciaran Murphy-Royal présentera l’article suivant :

" An optimized fluorescent probe for visualizing glutamate neurotransmission"

by Marvin, J.S., Borghuis, B.G., Cichon, J., Harnett, M.T, Akerboom, J., Gordus, A., Renninger, S.L., Chen, T.W., Bargmann, Cl., Orger, M.B,Schreiter, ER., Demb, JB., Gan, WB., Hires SA., Looger, LL.

Publié dans Nature Methods, 2013 Feb ; 10(2):162-70. doi: 10.1038/nmeth.2333.

Abstract :

We describe an intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) with signal-to-noise ratio and kinetics appropriate for in vivo imaging. We engineered iGluSnFR in vitro to maximize its fluorescence change, and we validated its utility for visualizing glutamate release by neurons and astrocytes in increasingly intact neurological systems. In hippocampal culture, iGluSnFR detected single field stimulus-evoked glutamate release events. In pyramidal neurons in acute brain slices, glutamate uncaging at single spines showed that iGluSnFR responds robustly and specifically to glutamate in situ, and responses correlate with voltage changes. In mouse retina, iGluSnFR-expressing neurons showed intact light-evoked excitatory currents, and the sensor revealed tonic glutamate signaling in response to light stimuli. In worms, glutamate signals preceded and predicted postsynaptic calcium transients. In zebrafish, iGluSnFR revealed spatial organization of direction-selective synaptic activity in the optic tectum. Finally, in mouse forelimb motor cortex, iGluSnFR expression in layer V pyramidal neurons revealed task-dependent single-spine activity during running.

MAINGRET Vincent   vincent.maingret @
Institut François Magendie
146 rue Léo Saignat
33077 Bordeaux Cedex

Vincent Maingret (vincent.maingret @

Journal Club Synapse

Le journal club synapse fait écho à la Journée Synapse, la réunion annuelle de l'axe synapse de la FBN

Ce journal club permet de discuter d'articles ayant trait au fonctionnement de la synapse, son développement, son rôle dans la dynamique des réseaux neuronaux, son implication dans la pathologie. Il a lieu tous les quinze jours, en Anglais, le Mardi à 13h30 à la CGFB, repas pizza offert....

For the newcomers, the Journal Club is an informal meeting where one of us presents a paper he/she thinks is really important in the field of synapse function and worth discussing for 30-40 minutes. As you know SFR Bordeaux Neuroscience will provide pizza for lunch!!