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Maciej M. Jankowski "Distribution of lymphocyte subsets following repeated intravenous administration of cocaine in rats: effects of pretreatment with haloperidol"

Abstract :

The distribution of immune cells in peripheral blood is commonly used diagnostic marker in humans and animals.
Cocaine is one of the most frequently abused illicit drugs worldwide and its use is associated with the increased susceptibility for human immunodeficiency virus (HIV) and hepatitis C virus (HCV) infections. Moreover, cocaine addicts show faster progression of HIV infection and increased incidence of acquired immunodeficiency syndrome (AIDS) or tuberculosis. Increased susceptibility for viral and bacterial infections suggests that cocaine affects the immune system. However, previous studies focused on the effects of cocaine on the lymphocyte subsets in humans failed to provide coherent conclusion that might be attributed to the uncontrolled dose range, duration, frequency and route of administration of cocaine, or multi-drug use in tested patients.
Cocaine suppresses the immune system via both central and peripheral mechanisms. The activation of hypothalamic-pituitary-adrenal axis and sympathetic nervous system are the major pathways through which cocaine modulates the immune system. We hypothesized that in addition to alterations in the neuroendocrine and sympathetic response, cocaine may also affect lymphocyte subsets by increased dopaminergic activity at both peripheral and central sites. At peripheral site cocaine may directly affect DA signaling between peripheral blood lymphocytes which express tyrosine hydroxylase, dopamine transporter (DAT) and DA receptors. Moreover, DA, DAT and DA receptors were found in the peripheral lymphoid tissues such as thymus or spleen.
In my talk I will discuss how I utilized the pattern of binge intravenous administration of cocaine to induce specific alterations in the distribution of lymphocyte subsets in peripheral blood of rats. I will also present the data from an acute experiment aimed to evaluate the possible involvement of dopamine receptors in the cocaine-induced alterations in the distribution of lymphocyte subsets in peripheral blood and spleen of rats.

Selected publications

Ignatowska-Jankowska, B., Jankowski, M.M., Swiergiel, A.H., 2011. Cannabidiol decreases body weight gain in rats: involvement of CB2 receptors. Neurosci. Lett. 490, 82-4.
Jankowski, M.M., Ignatowska-Jankowska, B., Glac, W., Swiergiel, A.H., 2010. Cocaine administration increases CD4/CD8 lymphocytes ratio in peripheral blood despite lymphopenia and elevated corticosterone. Int. Immunopharmacol. 10, 1229-34.
Ignatowska-Jankowska, B., Jankowski, M., Glac, W., Swiergiel, A.H., 2009. Cannabidiol-induced lymphopenia does not involve NKT and NK cells. J. Physiol. Pharmacol. (Suppl. 3) 60, 99-103.

Trojniar, W., Plucinska, K., Ignatowska-Jankowska, B., Jankowski, M., 2007. Damage to the nucleus accumbens shell but not core impairs ventral tegmental area stimulation-induced feeding. J. Physiol. Pharmacol. (Suppl. 3) 58, 63-71.

Véronique Deroche Gamonet du Neurocentre Magendie