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Emmanuel Valjent"Investigating Striatal Signaling through Cell-Type-Specific Identification"

Abstract :

Understanding how molecular signaling pathways participate in behavioral responses requires determining precisely in which neuronal populations they are activated.
The striatum is perhaps the most extensively studied region of the brain in terms of pharmacology and signaling pathways. However, most of these studies were performed using traditional approaches that examine an average of signaling events occurring in mixed populations of cells. The recent development of bacterial artificial chromosome (BAC) transgenic mice expressing a variety of reporters, epitope tagged-proteins or Cre recombinase driven by specific promoters, is a significant step forward to overcome this limitation. We took advantage of these mice to revisite the regulation of the mitogen-activated protein kinase (MAP-kinase)/extracellular signal-regulated kinase (ERK) pathway in the medium-sized spiny neurons (MSNs) of the striatum. Our results suggest that therapeutic agents and drugs of abuse produce a unique topography and cell-type specific regulation of the ERK cascade signaling in the mouse striatum.      

Selected publications

Bertran-Gonzalez J, Bosch C, Maroteaux M, Matamales M, Hervé D, Valjent E, Girault JA. Opposing patterns of signaling activation in dopamine D1 and D2 receptor-expressing striatal neurons in response to cocaine and haloperidol. J Neurosci. 2008 28(22):5671-85.

Valjent E, Bertran-Gonzalez J, Hervé D, Fisone G, Girault JA. Looking BAC at striatal signaling : cell-specific analysis in new transgenic mice. Trends Neurosci. 2009 32(10):538-47.

Valjent E, Bertran-Gonzalez J, Bowling H, Lopez S, Santini E, Matamales M, Bonito-Oliva A, Hervé D, Hoeffer C, Klann E, Girault JA, Fisone G. Haloperidol regulates the state of phosphorylation of ribosomal protein S6 via activation of PKA and phosphorylation of DARPP-32. Neuropsychopharmacology. 2011 36(12):2561-70.

Giovanni Marsicano