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David DiGregorio "Spatio-temporal filtering of information flow by sublinear synaptic integration within thin dendrites"

Abstract :


My laboratory is interested in the cellular and synaptic mechanisms influencing information flow through neuronal circuits. In principal neurons throughout the brain, dendrites have been shown to act as independent computational units through both supra-linear and sub-linear synaptic integration, often mediated by voltage-gated channels.
Dendritic integration of synaptic inputs in aspiny interneurons has received much less attention. We combine patch-clamp recordings, two-photon 2-photon-targeted electrical stimulation, glutamate uncaging, electron microscopy reconstructions, and numerical simulations to study the spatial and temporal distribution of synaptic integration and short-term plasticity of adult cerebellar interneurons (stellate cells). We find that that SCs use thin dendrites to spatially and temporally filter simultaneous GC inputs into the same dendrite, and suppress short-term facilitation at single synapses within the dendrites. These general cellular mechanisms result in a circularly symmetric weighting of incoming GC and inputs could influence the efficiency of patterned GC activation of Purkinje cells.

Selected publications

Submillisecond optical reporting of membrane potential in situ using a neuronal tracer dye. Bradley J, Luo R, Otis TS, DiGregorio DA.J Neurosci. 2009 Jul 22;29(29):9197-209.
Desensitization properties of AMPA receptors at the cerebellar mossy fiber granule cell synapse.DiGregorio DA, Rothman JS, Nielsen TA, Silver RA.J Neurosci. 2007 Aug 1;27(31):8344-57.
Lutz, C. T. Otis, V. DeSars, S. Charpak, D. DiGregorio, and V. Emiliani. Holographyc photolysis of caged neurotransmitters. Nature Methods, September Nature Methods 5, 821-827 (2008)

Scientific focus :

David DiGregorio est Lauréat ATIP en 2005 et ATIP Plus en 2008, il est responsable de l'équipe Fonction synaptiques et méthodes optiques au sein du laboratoire Physiologie Cérébrale (CNRS – Paris V). 

Valentin Nagerl