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Deniz Dalkara-Mourot et Alexandre Mourot"AAV mediated gene therapy approaches to retinal degeneration". & "Light at the end of the channel: Optical control of native and engineered ion channels"

Abstract :


Photochemical tools are transforming our ability to monitor and manipulate electrical activity and signaling in cells. Synthetic small molecule photoswitches can bestow light-sensitivity on native (1) or exogenously expressed proteins (2), including voltage-gated K+ and Na+ channels as well as ligand-gated glutamate and nicotinic receptors, allowing photocontrol of action potential firing and synaptic events. These photochemical tools have different capabilities and uses, but they all share in enabling precise and noninvasive exploration of neural function with light.

Selected publications

Fluorescent agonists for the Torpedo nicotinic acetylcholine receptor.Krieger F, Mourot A, Araoz R, Kotzyba-Hibert F, Molgó J, Bamberg E, Goeldner M.Chembiochem. 2008 May 5;9(7):1146-53.
Agonist- and competitive antagonist-induced movement of loop 5 on the alpha subunit of the neuronal alpha4beta4 nicotinic acetylcholine receptor.Mourot A, Bamberg E, Rettinger J.J Neurochem. 2008 Apr;105(2):413-24.
Changes in adeno-associated virus-mediated gene delivery in retinal degeneration.Kolstad KD, Dalkara D, Guerin K, Visel M, Hoffmann N, Schaffer DV, Flannery JG. Hum Gene Ther. 2010 May;21(5):571-8.
A novel adeno-associated viral variant for efficient and selective intravitreal transduction of rat Müller cells.Klimczak RR, Koerber JT, Dalkara D, Flannery JG, Schaffer DV. PLoS One. 2009 Oct 14;4(10):e7467.

Daniel Choquet