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Vincent Studer"Microfluidic tools for cell biology"

Abstract :


Recent trends in cell biology require advanced methods to apply spatially and temporally controlled stimulations to live cells and to quantitatively analyze their individual response at the subcellular or even at the molecular level. Moreover, high throughput methods to grasp the heterogeneity of cell populations are highly sought after. Methods to apply chemical stimulations, which are often based on the diffusion of solutes released by micropipettes, have long suffered from a poor spatial and temporal resolution. Only recently, advances in microfluidics have allowed complex laminar flows to be manipulated in networks of microchannels, in a possibly automated and parallelized manner and using minute amounts of reagents. Microfluidics is therefore considered a key technology for the development of quantitative cell-based assays and for experiments at a systems level. I will try to demonstrate its potential in cell biology by highlighting recent studies on bacterial population dynamics, yeast mating, neutrophil chemotaxis or embryonic development. In this presentation I will detail the main characteristics of microfluidic flows. I will give an overview of the most common fabrication techniques for microfluidic circuits and particularly those requiring very little equipment. Eventually I will describe ongoing microfluidic cell based assays in our lab.

Selected publications

Microfluidic droplet-based liquid-liquid extraction.
Mary P, Studer V, Tabeling P.
Anal Chem. 2008 Apr 15;80(8):2680-7.
     
Integrating whole transcriptome assays on a lab-on-a-chip for single cell gene profiling.
Bontoux N, Dauphinot L, Vitalis T, Studer V, Chen Y, Rossier J, Potier MC.Lab Chip. 2008 Mar;8(3):443-50.
     
Microfluidic stickers.
Bartolo D, Degré G, Nghe P, Studer V.
Lab Chip. 2008 Feb;8(2):274-9. Epub 2007 Nov 22.