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Hacène Boukari "Fluorescence Correlation Spectroscopy: A Quantitative Bioimaging Tool"

Abstract :


Fluorescence correlation spectroscopy (FCS) has emerged as a powerful quantitative bioimaging tool to characterize nanoscopic structures and to assess interactions of biomacromolecules in complex media –live cells, extracellular matrices, and solutions- sometimes at near single-molecule level.
In this talk, I will briefly introduce the technique and, then, describe the results of several studies where we have applied successfully the technique to quantify the oligomerization, diffusion, and binding of various in their respective host medium. Examples are the motility and binding of Glucocorticoids in the nucleus, the transport of HIV viruses in human cervical mucus, the depolymerization of microtubules due to novel cytotoxic peptides, the interactions of endophilin B1 and B2 with liposomes, and the diffusion of biomacromolecules in biogels. During the talk I will point out at the advantages as well as possible limitations of the technique, especially as related to the interpretation and modeling of the data obtained from complex media. Here, I discuss further several experimental setups, together with new approaches, that would allow us to decipher spatio-temporal interactions in these systems.

Selected publications

Boukari H, Sackett DL.
Fluorescence correlation spectroscopy and its application to the characterization of molecular properties and interactions.
Methods Cell Biol. 2008;84:659-78.
Boukari H, Sackett DL, Schuck P, Nossal RJ.
Single-walled tubulin ring polymers.
Biopolymers. 2007 Aug 5-15;86(5-6):424-36.
Michelman-Ribeiro A, Horkay F, Nossal R, Boukari H.
Probe diffusion in aqueous poly(vinyl alcohol) solutions studied by fluorescence correlation spectroscopy.
Biomacromolecules. 2007 May;8(5):1595-600. Epub 2007 Apr 19.

Scientific focus :

PhD in Chemical Physics: University of Maryland, College Park, USA, 1992.
Professional Appointments:
1999 – Now: Senior Research Scientist/Head of Microscopy Facilities, Laboratory of Integrative and Medical Biophysics, National Institutes of Health, Bethesda, Maryland, USA
1997-1999: Guest Researcher, National Institute of Standard and Technology, Gaithersburg, Maryland, USA.
1996-1997: Postdoctoral Fellow, University of Maryland, College Park, Maryland, USA.
1990-1996: Scientist, NASA/University of Maryland, College Park, Maryland, USA..

l'IFR, le CRI, l'IGF et le PCS.