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Marco Canepari"New insights in neuronal dendritic integration revealed by combined voltage and calcium imaging".

Abstract :

Processing of information in nerve cells and functional shaping of neuronal circuitries are governed by signal integration in the dendrites.
Dendritic integration is determined by the geometry of the dendrite, its intrinsic signalling capabilities and the spatio/temporal patterning of its input signals. In this talk I will present a recently developed approach of combined voltage and calcium imaging from the dendrites of individual neurons and the results of two studies from CA1 hippocampal pyramidal neurons and from cerebellar Purkinje neurons. In CA1 hippocampal pyramidal neurons, I will illustrate how the back-propagating action potentials are locally modulated by synaptic potentials in patterns of activity that induce long-term potentiation of synaptic signals. In cerebellar Purkinje I will show how parallel fibre synaptic potentials can locally excite the dendrites and how the calcium signals evoked by these depolarisations can induce long-term plasticity. The presentation will include a view of the future prospective in the utilization of this novel optical approach.

Short CV
Date of Birth: May 29th 1970
University degree in Physics: University of Genoa, Italy, June 1994 (110/100 cum laude). Thesis: ”Recording techniques from small biological neural networks.” - Supervisor Prof. V.Torre. PhD in Biophysics: SISSA/ISAS, Trieste, Italy, February 1999. Thesis: ”Intrinsic variability and short-term changes in synaptic transmission in the rat hippocampal CA3 region.” - Supervisor Prof. Enrico Cherubini, Co-Supervisor Prof. Alessandro Treves.
Principal professional experiences:
March 1999 – March 2005: Postdoctoral associate at the National Institute for Medical research (NIMR), London, UK. – Principal Investigator: Dr. David Ogden.
April 2005 – December 2006: Senior postdoctoral associate at Yale University School of Medicine, New haven, USA. – Principal Investigator: Dr. Dejan Zecevic.
March 2006 – Today: Research associate at the Biozentrum, University of Basel, CH.

Selected publications

Canepari M, Djurisic M, Zecevic D (2007) Dendritic signals from rat hippocampal CA1 pyramidal neurons during coincident pre- and post-synaptic activity: a combined voltage- and calcium-imaging study. Journal of Physiology, 580: 463-484.
Canepari M, Odgen D (2006) Kinetic, pharmacological and activity-dependent separation of two Ca2+ signalling pathways mediated by type 1 metabotropic glutamate receptors in rat Purkinje neurons. Journal of Physiology, 573: 65-82.
Canepari M, Auger C, Ogden D (2004) Ca2+ ion permeability and single channel properties of the parallel fibre metabotropic slow EPSC of rat Purkinje neurons. Journal of Neuroscience, 24: 3563-3573.