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Advanced Imaging Methods for Cellular Neuroscience

Cajal Training Courses 2018, Bordeaux Neurocampus

Du 10 septembre 2018 au 28 septembre 2018

Advanced Imaging Methods for Cellular Neuroscience:
The students will obtain with this course hands-on experience with innovative techniques expected to be central in cellular neuroscience in the coming decade. They will include in vitro and in vivo gene transfer, cell and tissue imaging by confocal imaging and light sheet microscopy, photomanipulation in living tissue and optophysiology, patch clamp electrophysiology, imaging of proteins and lipids by super-resolution microscopy (STED and PALM/STORM), single-particle tracking methodologies, correlative light electron microscopy (CLEM), live imaging of protein interactions (FRET, FLIM). Rodent and human model systems (iPSCs, organoids) are central, and successful invertebrate models such as Drosophila and C. elegans will be also available.

 Lectures open / CGFB conference room / free entry

Advanced Imaging Methods for Cellular Neuroscience

Tuesday 11

9h00: Erika Holzbaur - Autophagy dynamics in neuronal homeostasis and neuro-degeneration
10h15: Christian Rosenmund - Investigation of mechanisms of synaptic vesicle exocytosis using optical methods
11h30: Eric Jorgensen – Resolving time in electron microscopy

Wednesday 12
9h00: Gary Lewin - Exploring the biology of sensory transduction with extremophile mole-rats
10h15: Shigeki Watanabe - Spatial and temporal control of synaptic transmission
11h30: Janine Kirstein - Chaperone-mediated remodeling of amyloid proteins
12h15: Marius Wernig - Properties of neurons derived from human induced pluripotent stem cells

Saturday 15
9h00: Silvio Rizzoli - Super-resolution imaging of synaptic composition 
10h15: Maxime Cazorla - Reconstituting brain networks on-a-chip to study intracellular dynamics
11h30: Cyril Hanus - Compartmentalized processing in the dendritic secretory pathway
12h15: Guillaume Van Niel - Live tracking of exosome secretion and communication

Monday 17
9h00: Volke Haucke - Mechanisms of presynapse function and assembly
10h15: Daniela Dieterich- Probing translational capacities with a click
11h30: Gregory Giannone - Unveil the nano-scale organization of β3-integrin at dendritic spines
12h15: Andrew Plested - Optical methods to study glutamate receptor biophysics

Thursday 20
9h00: Stephan Sigrist – From proteins to behavior: shedding light on synapse organisation
10h15: Valentin Naegerl - Super-resolution microscopy for neuroscience: new approaches and applications
11h30: Patrik Vestreken - Synaptic protein turnover in Parkinson’s disease

Friday 21
9h00: Mike Fainzilber - The Importins of Local Translation in Neuronal Growth and Regeneration 
10h15: Robin Hiesinger - Live imaging of axon terminal development and maintenance in Drosophila brains
11h30: Alexander Walter - Analysis of SV release site coupling distances to voltage gated Ca2+ channels at active zones of the Drosophila neuromuscular junction
12h15: David Owald - Spontaneous Ca2+ and voltage fluctuations in the Drosophila brain

Saturday 22
9h00: Frédéric Saudou - Huntingtin: Linking Fast Axonal Transport, Energy Supply and Neurotrophin Signaling to Neurodegeneration
10h15: Anna Fejtova - CtBP1 promotes synaptic vesicle cycling by formation of permissive lipid environment for compensatory endocytosis
11h30: Ingrid Chamma - Super-resolution imaging of synaptic adhesion protein dynamics and organization
12h15: Helge Ewers - Nanoscale organization and function of the cytoskeleton

Wednesday 25
9h00: Gianpietro Schiavo - Axonal transport in health and disease
10h15: Laura Andreae - STORM imaging of synaptic vesicle pools during neuronal development
11h30: Klaus Nave - Myelination and the glial support of axonal energy metabolism

Thursday 27
9h00: Daniel Choquet - Nanoscale dynamic organization of receptors and synaptic function
11h30: Ira Milosevic - Regulation of vesicle acidification at the neuronal synapse
12h15: Etienne Herzog – Sorting and imaging specific synaptosomes from the mouse brain 

Applications deadline: 14 May 2018
Date and Location: 10-28 September 2018

Bordeaux Neurocampus, France

Course directors:
Volker Haucke, Leibniz Institute for Molecular Pharmacology (FMP), Berlin, Germany
Britta Eickholt, Charité – Universitätsmedizin Berlin, Germany
David Perrais, IINS, CNRS - University of Bordeaux, France

Confirmed Speakers:
Vivian Budnik, University of Massachusetts Medical School, USA
Pietro De Camilli, Yale School of Medicine, USA
Mike Fainzilber, Weizmann Institute of Science, Israel
Erika Holzbaur, University of Pennsylvania, PA, USA
Erik Jorgensen, University of Utah, Salt Lake City, USA
Gary Lewin, Max Delbrück Center for Molecular Medicine, Germany
Klaus Nave, Max Planck Institute, Germany
Silvio O. Rizzoli, University of Göttingen Medical Center, Germany
Frédéric Saudou, Grenoble University Hospital CHUGA, France
Gipi Schiavo, University College London, UK
Stephan Sigrist, Freie Universitat, Berlin, Germany
Patrik Verstreken, VIB-KU Leuven Center for Brain & Disease Research, Belgium

This course is organised in partnership with the Bordeaux Imaging Centre.